Evidence linking exposure of fish primary macrophages to antibiotics activates the NF-kB pathway

Qiu, Wenhui1,2; Hu, Jiaqi1,2; Magnuson, Jason T.3; Greer, Justin3; Yang, Ming4; Chen, Qiqing5; Fang, Meijuan1,2; Zheng, Chunmiao1,2; Schlenk, Daniel3

2020

10.1016/j.envint.2020.105624

Environment International   影响因子和分区

18736750

1873-6750

Low doses of antibiotics are ubiquitous in the marine environment and may exert negative effects on non-target aquatic organisms. Using primary macrophages of common carp, we investigated the mechanisms of action following exposure to several common antibiotics; cefotaxime, enrofloxacin, tetracycline, sulfamonomethoxine, and their mixtures, and explored the immunomodulatory effects associated with the nuclear factor-κB (NF-κB) signaling pathway. A KEGG pathway analysis was conducted using the sixty-six differentially expressed genes found in all treatments, and showed that exposure to 100 μg/L of antibiotics could affect regulation of the NF-κB signaling pathway, suggesting that activation of NF-κB is a common response in all four classes of antibiotics. In addition, the four antibiotics induced nf-κb and NF-κB-associated cytokines expression, as verified by qPCR, however, these induction responses by four antibiotics were minor when compared to the same concentration of LPS treatment (100 μg/L). Antagonists of NF-κB blocked many of the immune effects of the antibiotics, providing evidence that NF-κB pathways mediate the actions of all four antibiotics. Moreover, exposure to environmentally relevant, low levels (0.01–100 μg/L) of antibiotics induced a NF-κB-mediated immune response, including endogenous generation of ROS, activity of antioxidant enzymes, as well as expression of cytokine and apoptosis. Moreover, exposure to mixtures of antibiotics presented greater effects on most tested immunological parameters than exposure to a single antibiotic, suggesting additive effects from multiple antibiotics in the environment. This study demonstrates that exposure of fish primary macrophages to low doses of antibiotics activates the NF-kB pathway.
© 2020 The Authors

Antibiotics Nuclear factor-kappa B Immunomodulatory Macrophages

EI ; SCI

英语

第一 ; 通讯

Southern University of Science and Technology[G01296001] ; [2016YFC0402806] ; State Environmental Protection Key Laboratory of Sources and Control of Air Pollution Complex[] ; [JCYJ20180302180205159] ; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology[2017B030301012] ; Leading Talents Program of Guangdong Province[] ; National Natural Science Foundation of China[21707064]

Environmental Sciences & Ecology

Environmental Sciences

WOS:000522749900031

Elsevier Ltd

20201008264310

Activation analysis ; Cell death ; Fish ; Macrophages ; Mixtures ; Polymerase chain reaction

Bioengineering and Biology:461 ; Biochemistry:801.2

ENVIRONMENT/ECOLOGY

EV Compendex

1.State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen; 518055, China
2.Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen; 518055, China
3.Department of Environmental Sciences, University of California, Riverside; CA; 92521, United States
4.School of Environmental and Chemical Engineering, Shanghai University, Shanghai; 200444, China
5.State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai; 200062, China

Qiu, Wenhui,Hu, Jiaqi,Magnuson, Jason T.,et al. Evidence linking exposure of fish primary macrophages to antibiotics activates the NF-kB pathway[J]. Environment International,2020,138.

Qiu, Wenhui.,Hu, Jiaqi.,Magnuson, Jason T..,Greer, Justin.,Yang, Ming.,...&Schlenk, Daniel.(2020).Evidence linking exposure of fish primary macrophages to antibiotics activates the NF-kB pathway.Environment International,138.

Qiu, Wenhui,et al."Evidence linking exposure of fish primary macrophages to antibiotics activates the NF-kB pathway".Environment International 138(2020).