南方科技大学知识苑(SUSTech KC): PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness

题名	PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness
作者	Qi, Lijuan 1; Liu, Shan 1; Li, Cong 1; Fu, Jingying 1; Jing, Yanjun 2; Cheng, Jinkui 1; Li, Hong 1; Zhang, Dun 1; Wang, Xiaoji 1; Dong, Xiaojing 1; Han, Run 1; Li, Bosheng3 ; Zhang, Yu 4,5; Li, Zhen 1; Terzaghi, William 6; Song, Chun-Peng 7; Lin, Rongcheng 2; Gong, Zhizhong 1; Li, Jigang 1
通讯作者	Li, Jigang
发表日期	2020-03-02
DOI	10.1016/j.molp.2020.02.001
发表期刊	Molecular Plant 影响因子和分区
ISSN	1674-2052
EISSN	1752-9867
卷号	13 期号:3页码:414-430
摘要	PHYTOCHROME-INTERACTING FACTORS (PIFs) are a group of basic helix-loop-helix transcription factors that can physically interact with photoreceptors, including phytochromes and cryptochromes. It was previously demonstrated that PIFs accumulated in darkness and repressed seedling photomorphogenesis, and that PIFs linked different photosensory and hormonal pathways to control plant growth and development. In this study, we show that PIFs positively regulate the ABA signaling pathway during the seedling stage specifically in darkness. We found that PIFs positively regulate ABI5 transcript and protein levels in darkness in response to exogenous ABA treatment by binding directly to the G-box motifs in the ABI5 promoter. Consistently, PIFs and the G-box motifs in theABI5 promoter determineABI5 expression in darkness, and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark. Moreover, we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9, and that this interaction is not regulated by ABA. Further analyses showed that PYL8 and PYL9 promote PIF4 protein accumulation in the dark and enhance PIF4 binding to the ABI5 promoter, but negatively regulate PIF4-mediated ABI5 activation. Taken together, our data demonstrate that PIFs interact with ABA receptors to orchestrate ABA signaling in darkness by controlling ABI5 expression, providing new insights into the pivotal roles of PIFs as signal integrators in regulating plant growth and development.
关键词	Arabidopsis transcription factor PIFs ABA ABI5 darkness
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	Ministry of Agriculture of China[2019ZX08010003-002-005]
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000518446000008
出版者	CELL PRESS
来源库	Web of Science
引用统计	被引频次[WOS]：76
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/104543
专题	南方科技大学-北京大学植物与食品联合研究所生命科学学院_生物系
作者单位	1.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 3.Southern Univ Sci & Technol, Inst Plant & Food Sci, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China 4.Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA 5.ARS, Plant Gene Express Ctr, USDA, Albany, CA 94710 USA 6.Wilkes Univ, Dept Biol, Wilkes Barre, PA 18766 USA 7.Henan Univ, Collaborat Innovat Ctr Crop Stress Biol, Inst Plant Stress Biol, Kaifeng 475001, Peoples R China
推荐引用方式 GB/T 7714	Qi, Lijuan,Liu, Shan,Li, Cong,et al. PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness[J]. Molecular Plant,2020,13(3):414-430.
APA	Qi, Lijuan.,Liu, Shan.,Li, Cong.,Fu, Jingying.,Jing, Yanjun.,...&Li, Jigang.(2020).PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness.Molecular Plant,13(3),414-430.
MLA	Qi, Lijuan,et al."PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness".Molecular Plant 13.3(2020):414-430.