南方科技大学知识苑(SUSTech KC): Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major Mycobacterium Species

题名	Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major Mycobacterium Species
作者	Xiao, Guohui 1,2; He, Xing 1; Zhang, Su 1; Liu, Yaya 1; Liang, Zhihang 3; Liu, Houming 1; Zhang, Juanjuan 1; Ou, Min 1; Cai, Shuhao 1; Lai, Wenjie 1; Zhang, Tianyu 2; Ren, Lili 4,5; Zhang, Guoliang 1,3
通讯作者	Zhang, Guoliang
发表日期	2020-02
DOI	10.1128/JCM.01368-19
发表期刊	JOURNAL OF CLINICAL MICROBIOLOGY 影响因子和分区
ISSN	0095-1137
EISSN	1098-660X
卷号	58 期号:2
摘要	Mycobacterium tuberculosis infection and nontuberculous mycobacteria (NTM) infections exhibit similar clinical symptoms; however, the therapies for these two types of infections are different. Therefore, the rapid and accurate identification of M. tuberculosis and NTM species is very important for the control of tuberculosis and NTM infections. In the present study, a Cas12a/guide RNA (gRNA)-based platform was developed to identify M. tuberculosis and most NTM species. By designing species-specific gRNA probes targeting the rpo8 sequence, a Cas12a/gRNA-based platform successfully 11 identified M. tuberculosis and six major NTM species (Mycobacterium abscessus, Mycobacterium intracellulare, Mycobacterium avium, Mycobacterium kansasii, Mycobacterium gordonae, and Mycobacterium fortuitum) without cross-reactivity. In a blind assessment, a total of 72 out of 73 clinical Mycobacterium isolates were correctly identified, which is consistent with previous rpo8 sequencing results. These results suggest that the Cas12a/gRNA-based platform is a promising tool for the rapid, accurate, and cost-effective identification of both M. tuberculosis and NTM species.
关键词	Mycobacterium tuberculosis nontuberculous mycobacteria CRISPR/Cas12a gRNA probe fluorescence identification biosensing
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	State Key Laboratory of Respiratory Diseases Open Project[SKLRD-OP-201919]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000515495700012
出版者	AMER SOC MICROBIOLOGY
ESI学科分类	MICROBIOLOGY
来源库	Web of Science
引用统计	被引频次[WOS]：12
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/104590
专题	南方科技大学第二附属医院南方科技大学第一附属医院
作者单位	1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Guangdong Key Lab Emerging Infect Dis, Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China 2.Chinese Acad Sci, GIBH, State Key Lab Resp Dis, Guangzhou, Peoples R China 3.Guangdong Med Univ, Sch Basic Med Sci, Dongguan, Peoples R China 4.Chinese Acad Med Sci, IPB, Beijing, Peoples R China 5.Peking Union Med Coll, Beijing, Peoples R China
第一作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
通讯作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Xiao, Guohui,He, Xing,Zhang, Su,et al. Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major Mycobacterium Species[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2020,58(2).
APA	Xiao, Guohui.,He, Xing.,Zhang, Su.,Liu, Yaya.,Liang, Zhihang.,...&Zhang, Guoliang.(2020).Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major Mycobacterium Species.JOURNAL OF CLINICAL MICROBIOLOGY,58(2).
MLA	Xiao, Guohui,et al."Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major Mycobacterium Species".JOURNAL OF CLINICAL MICROBIOLOGY 58.2(2020).