The Length and Distribution of Plasma Cell-Free DNA Fragments in Stroke Patients

Cui, Xiaofang1,2; Du, Shiyi3; Liu, Houlin4; Liu, Ju4; Wu, Qingjian4; Huo, Qing5; Qi, Yanwei6; Qin, Xiao1,7; Yang, Yan1; Li, Weiyang1,7

2020-01-30

10.1155/2020/9054196

Biomed Research International   影响因子和分区

2314-6133

2314-6141

A number of studies have shown that plasma cell-free DNA is closely related to the risk of stroke, but the fragmentation status of plasma cell-free DNA and its clinical application value in ischemic stroke are still unclear. In this study, 48 patients with new ischemic stroke and 20 healthy subjects were enrolled. The second-generation high-throughput sequencing technique was used to study the plasma cell-free fragment length and regional distribution of the subjects. As noted in our results, the ratio of plasma cell-free DNA fragments in the disease group was significantly greater than that of the healthy group in the 300-400 bp range; conversely for fragments at the 75-250 bp range, the ratio of plasma cell-free DNA fragments in the patient group was apparently lower than that of the healthy group. In-depth analysis of the proportion of fragments distributed on each component of the genome was carried out. Our results recorded that the plasma cell-free DNA fragments in the disease group were inclined to the EXON, CpG islands, and ALU regions in contrast to that of the healthy group. In particular, fragments within the 300-400 bp range of the disease group were enrichment in the regions of EXON, INTRON, INTERGENIC, LINE, Fragile, ALU, and CpG islands. In summary, our findings suggested that the intracellular DNA degradation profiles could be applied to distinguish the stroke group and the healthy group, which provided a theoretical basis for the clinical diagnosis and prognosis of stroke by profiling the characteristic of plasma cell-free DNA fragments.

SCI

英语

其他

Supporting Fund for Teachers Research of Jining Medical University[JY2017JS004]

Biotechnology & Applied Microbiology ; Research & Experimental Medicine

Biotechnology & Applied Microbiology ; Medicine, Research & Experimental

WOS:000514387200001

HINDAWI LTD

Web of Science

1.Jining Med Univ, Jining 272067, Shandong, Peoples R China
2.Jining Med Univ, Shandong Key Lab Behav Med, Sch Mental Hlth, Jining 272067, Shandong, Peoples R China
3.Guangzhou Root Path Genom Inc, Guangzhou 510006, Peoples R China
4.Jining 1 Peoples Hosp, Jining 272011, Shandong, Peoples R China
5.Southern Univ Sci & Technol, Shenzhen 518055, Peoples R China
6.City Univ Hong Kong, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
7.Jining Med Univ, Collaborat Innovat Ctr Birth Defect Res & Transfo, Jining 272067, Shandong, Peoples R China

Cui, Xiaofang,Du, Shiyi,Liu, Houlin,et al. The Length and Distribution of Plasma Cell-Free DNA Fragments in Stroke Patients[J]. Biomed Research International,2020,2020.

Cui, Xiaofang.,Du, Shiyi.,Liu, Houlin.,Liu, Ju.,Wu, Qingjian.,...&Li, Weiyang.(2020).The Length and Distribution of Plasma Cell-Free DNA Fragments in Stroke Patients.Biomed Research International,2020.

Cui, Xiaofang,et al."The Length and Distribution of Plasma Cell-Free DNA Fragments in Stroke Patients".Biomed Research International 2020(2020).