Identification of the circRNA-miRNA-mRNA regulatory network of Hsp90 inhibitor-induced cell death in colorectal cancer by integrated analysis

Liu, Kaisheng1; Guo, Yaomin1; Zheng, Kai2; Zou, Chang1; Wu, Haixiong1; Wang, Shaoxiang2; Ou, Ling1; Wang, Yifei3; Huang, Bowan4; Wang, Xiao1

2020-02-15

10.1016/j.gene.2019.144232

GENE   影响因子和分区

0378-1119

1879-0038

Colorectal cancer (CRC) is a global disease with high incidence and mortality rate. Hsp90 inhibitors induce cell death in various cancers, including CRC. However, the underlying mechanisms need to be clarified further. In this study, Caco-2 cells were treated with 0.25 mu M SNX-2112, an Hsp90 inhibitor, for 48 h; subsequently, whole-transcriptome sequencing was performed. At the mRNA level in SNX-2112-treated Caco-2 cells, 1588 genes were upregulated, and 433 genes were downregulated. Six genes were found to be associated with necroptosis and apoptosis, and these 6 upregulated genes were validated by RT-qPCR. Hundred and six miRNAs were upregulated, and 48 miRNAs were downregulated in SNX-2112-treated Caco-2 cells. Eleven downregulated miRNAs were found to interact with the 6 upregulated genes. Moreover, 676 circRNAs were upregulated, and 291 circRNAs were downregulated in SNX-2112-treated Caco-2 cells. Among them, 126 circRNAs were found to be the target of the 11 downregulated miRNAs. The circRNA-miRNA-mRNA regulatory network of Hsp90 inhibitor-induced cell death in colorectal cancer was constructed. This regulatory network extends the underlying mechanism of Hsp90 and improves our understanding of Hsp90 inhibitors as potential targeted therapeutic agents.

Colorectal cancer SNX-2112 Cell death mRNA miRNA circRNA

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[81802749]

Genetics & Heredity

Genetics & Heredity

WOS:000508746800001

ELSEVIER

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Southern Univ Sci & Technol,Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
2.Shenzhen Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Shenzhen 518060, Guangdong, Peoples R China
3.Jinan Univ, Coll Life Sci & Technol, Inst Biomed, Guangzhou 510632, Guangdong, Peoples R China
4.Cent Peoples Hosp Zhanjiang, Dept Anesthesiol, Zhanjiang 524000, Guangdong, Peoples R China

Liu, Kaisheng,Guo, Yaomin,Zheng, Kai,et al. Identification of the circRNA-miRNA-mRNA regulatory network of Hsp90 inhibitor-induced cell death in colorectal cancer by integrated analysis[J]. GENE,2020,727.

Liu, Kaisheng.,Guo, Yaomin.,Zheng, Kai.,Zou, Chang.,Wu, Haixiong.,...&Wang, Xiao.(2020).Identification of the circRNA-miRNA-mRNA regulatory network of Hsp90 inhibitor-induced cell death in colorectal cancer by integrated analysis.GENE,727.

Liu, Kaisheng,et al."Identification of the circRNA-miRNA-mRNA regulatory network of Hsp90 inhibitor-induced cell death in colorectal cancer by integrated analysis".GENE 727(2020).