Metagenomic insights into the profile of antibiotic resistomes in a large drinking water reservoir

Dang, Chenyuan1,2; Xia, Yu2; Zheng, Maosheng3; Liu, Tang1; Liu, Wen1; Chen, Qian1; Ni, Jinren1

2020

10.1016/j.envint.2019.105449

Environment International   影响因子和分区

18736750

1873-6750

Reservoirs play a vital role in the control and management of surface water resources. However, the long water residence time in the reservoir potentially increases the storage and accumulation of antibiotic resistant genes (ARGs). The full profiles and potential health risks of antibiotic resistomes in reservoirs are largely unknown. In this study, we investigated the antibiotic resistomes of water and sediment during different seasons in the Danjiangkou Reservoir, which is one of the largest reservoirs in China, using a metagenomic sequencing approach. A total of 436 ARG subtypes belonging to 20 ARG types were detected from 24 water and 18 sediment samples, with an average abundance of 0.138 copies/cell. The overall ARG abundance in the sediment was higher than that in the water, and bacitracin and vancomycin resistance genes were the predominant ARG types in the water and sediment, respectively. The overall ARG abundance in the dry season was higher than that in the wet season, and a significant difference in ARG subtype compositions was observed in water, but not in the sediment, between the different seasons. The potential horizontal gene transfer frequency in the water was higher than that in the sediment, and the ARGs in water mainly came from the sediment upstream of the reservoir. The metagenomic assembly identified 14 contigs as ARG-carrying pathogens including Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa, and 3 of 14 carried virulence factors. Overall, the potential public health risks posed by resistomes in the water of the Danjiangkou Reservoir were higher in the dry season than in the wet season. Based on these results, strategies including sediment control and pathogen monitoring are suggested for water safety management in drinking water reservoirs.
© 2019

Antibiotic resistome Metagenomic sequencing Drinking water source The Danjiangkou Reservoir ARG host tracking

SCI ; EI

英语

其他

China Postdoctoral Science Foundation[2019M661148] ; National Natural Science Foundation of China[51721006]

Environmental Sciences & Ecology

Environmental Sciences

WOS:000512533700038

Elsevier Ltd

20200207994861

Antibiotics ; Drought ; Escherichia coli ; Gene transfer ; Genes ; Health risks ; Potable water ; Public risks ; Reservoir management ; Sediments ; Surface water resources ; Surface waters

Reservoirs:441.2 ; Water Resources:444 ; Surface Water:444.1 ; Bioengineering and Biology:461 ; Soil Mechanics and Foundations:483 ; Petroleum Deposits : Development Operations:512.1.2

ENVIRONMENT/ECOLOGY

Web of Science

1.The Key Laboratory of Water and Sediment Sciences, Ministry of Education, College of Environmental Sciences and Engineering, Peking University, Beijing; 100871, China
2.Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, China
3.College of Environmental Science and Engineering, North China Electric Power University, The Key Laboratory of Resources and Environmental Systems Optimization, Ministry of Education, Beijing; 102206, China

Dang, Chenyuan,Xia, Yu,Zheng, Maosheng,et al. Metagenomic insights into the profile of antibiotic resistomes in a large drinking water reservoir[J]. Environment International,2020,136.

Dang, Chenyuan.,Xia, Yu.,Zheng, Maosheng.,Liu, Tang.,Liu, Wen.,...&Ni, Jinren.(2020).Metagenomic insights into the profile of antibiotic resistomes in a large drinking water reservoir.Environment International,136.

Dang, Chenyuan,et al."Metagenomic insights into the profile of antibiotic resistomes in a large drinking water reservoir".Environment International 136(2020).