Circular RNA MAN2B2 promotes cell proliferation of hepatocellular carcinoma cells via the miRNA-217/MAPK1 axis

Fu, Xiaoying1,2; Zhang, Juanjuan1; He, Xing1; Yan, Xu1; Wei, Jian1; Huang, Min1; Liu, Yaya1; Lin, Jianwei3; Hu, Hongxing1; Liu, Lei1

2020

10.7150/jca.36500

Journal of Cancer   影响因子和分区

1837-9664

The increasing incidence of hepatocellular carcinoma (HCC) is a major challenge worldwide. In the past few years, an increasing number of studies have suggested that circular RNAs (circRNAs) play an important role in the development of human tumors, including HCC, but our understanding of their function is still limited. In this study, we investigated differences in the expression of circRNA MAN2B2 (circMAN2B2) in hepatocellular tissues and paired normal tissues. We found that knockdown of circMAN2B2 expression in the HCC cell lines Hep-G2 and Huh-7 significantly inhibited cell proliferation by sponging (miRNA) miR-217 and inhibiting its function. Through a series of experiments, we also demonstrated that miR-217 functioned as a tumor suppressor molecule in HCC cells and regulated the expression of mitogen-activated protein kinase 1 (MAPK1). Restoration of MAPK1 rescued repression of cell proliferation induced by circMAN2B2 knockdown. In summary, our study indicated that circMAN2B2 acted as an onco-miRNA in HCC by sponging miRNA-217 to promote MAPK1 expression.

hepatocellular carcinoma circMAN2B2 miR-217 MAPK1 circular RNA

SCI

英语

第一 ; 通讯

Shenzhen science and technology project[JCYJ20170307095236069]

Oncology

Oncology

WOS:000518775100025

IVYSPRING INT PUBL

Web of Science

1.Southern Univ Sci & Technol, Affiliated Hosp 2, Shenzhen Peoples Hosp 3, Shenzhen 518039, Peoples R China
2.Shenzhen Univ, Shenzhen 518039, Peoples R China
3.Shenzhen Peoples Hosp, Shenzhen 518039, Peoples R China

Fu, Xiaoying,Zhang, Juanjuan,He, Xing,et al. Circular RNA MAN2B2 promotes cell proliferation of hepatocellular carcinoma cells via the miRNA-217/MAPK1 axis[J]. Journal of Cancer,2020,11(11):3318-3326.

Fu, Xiaoying.,Zhang, Juanjuan.,He, Xing.,Yan, Xu.,Wei, Jian.,...&Liu, Lei.(2020).Circular RNA MAN2B2 promotes cell proliferation of hepatocellular carcinoma cells via the miRNA-217/MAPK1 axis.Journal of Cancer,11(11),3318-3326.

Fu, Xiaoying,et al."Circular RNA MAN2B2 promotes cell proliferation of hepatocellular carcinoma cells via the miRNA-217/MAPK1 axis".Journal of Cancer 11.11(2020):3318-3326.