Contamination profile of antibiotic resistance genes in ground water in comparison with surface water

Wu, Dai-Ling1,2; Zhang, Min1,2; He, Lu-Xi1,2; Zou, Hai-Yan1,2; Liu, You-Sheng1,2; Li, Bei-Bei1,2; Yang, Yuan-Yuan1,2; Liu, Chongxuan3; He, Liang-Ying1,2; Ying, Guang-Guo1,2

2020

10.1016/j.scitotenv.2020.136975

Science of the Total Environment   影响因子和分区

18791026

1879-1026

Dissemination of antibiotic resistance genes (ARGs) in the water environment has become an increasing concern. There have been many reports on ARGs in surface water, but little is known about ARGs in groundwater. In this study, we investigated the profiles and abundance of ARGs in groundwater in comparison with those in surface water of Maozhou River using high-throughput quantitative PCR (HT-qPCR). Totally 127 ARGs and 10 MGEs were detected by HT-qPCR, and among them the sulfonamides, multidrug and aminoglycosides resistance genes were the dominant ARG types. According to the results of HT-qPCR, 18 frequently detected ARGs conferring resistance to 6 classes of antibiotics and 3 MGEs were further quantified by qPCR in the wet season and dry season. The absolute abundance ranged from 1.23 × 10⁵ to 8.89 × 10⁶ copies/mL in wet season and from 8.50 × 10² to 2.65 × 10⁶ copies/mL in the dry season, with sul1 and sul2 being the most abundant ARGs. The absolute abundance of ARGs and MGEs has no significant difference between the wet season and dry season while the diversity of ARGs in the dry season was higher than that in the wet season (p < 0.05). Totally 141 and 150 ARGs were detected in the water and sediments of Maozhou River, respectively. A total of 116 ARGs were shared among the groundwater, river water, and sediment, which accounted for 67.1% of all detected genes. Redundancy analysis further demonstrated that the environmental factors contributed 70.7% of the total ARG variations. The findings of large shared ARGs, abundant Total Coliforms and large wastewater burden in the groundwater provide a clear evidence that anthropogenic activities had a significant impact on groundwater.
© 2020 Elsevier B.V.

Groundwater Antibiotic resistance ARGs MGEs Surface water

SCI ; EI

英语

其他

National Natural Science Foundation of China[41703117] ; National Natural Science Foundation of China[]

Environmental Sciences & Ecology

Environmental Sciences

WOS:000518409900035

Elsevier B.V.

20200508111085

Amides ; Antibiotics ; Drought ; Genes ; Groundwater ; Polymerase chain reaction ; Rivers ; Sulfur compounds ; Surface waters

Water Resources:444 ; Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Biochemistry:801.2 ; Organic Compounds:804.1

ENVIRONMENT/ECOLOGY

Web of Science

1.SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou; 510006, China
2.School of Environment, South China Normal University, University Town, Guangzhou; 510006, China
3.School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen; 518055, China

Wu, Dai-Ling,Zhang, Min,He, Lu-Xi,et al. Contamination profile of antibiotic resistance genes in ground water in comparison with surface water[J]. Science of the Total Environment,2020,715.

Wu, Dai-Ling.,Zhang, Min.,He, Lu-Xi.,Zou, Hai-Yan.,Liu, You-Sheng.,...&Ying, Guang-Guo.(2020).Contamination profile of antibiotic resistance genes in ground water in comparison with surface water.Science of the Total Environment,715.

Wu, Dai-Ling,et al."Contamination profile of antibiotic resistance genes in ground water in comparison with surface water".Science of the Total Environment 715(2020).