Development of a new simple mitochondria-targeted fluorescent K⁺ sensor and the application in high-throughput monitoring K⁺ fluxes

Ning, Juewei1,2; Tian, Yanqing2

2020

10.1016/j.snb.2020.127659

SENSORS AND ACTUATORS B-CHEMICAL   影响因子和分区

0925-4005

0925-4005

A new simple mitochondria-targeted fluorescent potassium ion (K⁺) sensor (NK1) was synthesized and investigated. The synthetic route of this sensor was much more concise than the previously reported mitochondrial-targeting KS6, enabling the potential broad applications of this sensor NK1. NK1 displays good responses to K⁺ with broad detection range from 10 to 500 mM, short response time (0.2 s), high sensitivity with 160 folds’ fluorescence enhancement at 1000 mM K⁺, a large dissociation constant (Kd about 200 mM), and minimum responses to physiological pH (5.0–9.0) as well as other biologically relevant metal ions. K⁺ fluxes (efflux and influx) in the mitochondria of living cells were observed upon manipulation with ionophores (nigericin or ionomycin) and various concentrations of KCl both under confocal microscope and especially microplate reader for first time high-throughput in-situ monitoring of mitochondrial K⁺ fluxes. The development of easily synthesized NK1 with excellent performance will be greatly beneficial for ion channel relative drug screening and the investigation of mitochondrial potassium channels as well as mitochondrial metabolism.
© 2020 Elsevier B.V.

Potassium ion sensor Mitochondria targeting K+ fluxes High-throughput monitoring

SCI ; EI

英语

通讯

Shenzhen Fundamental Research and Discipline Layout project[Y01256114] ; National Natural Science Foundation of China[21574061] ; [G01256018]

Chemistry ; Electrochemistry ; Instruments & Instrumentation

Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation

WOS:000508110400023

Elsevier B.V.

20200207990407

Chlorine compounds ; Dissociation ; Fluorescence ; Ionophores ; Metal ions ; Molecular biology ; pH ; Physiological models ; Potassium ; Potassium compounds

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Metallurgy:531.1 ; Alkali Metals:549.1 ; Light/Optics:741.1 ; Chemistry, General:801.1 ; Chemical Reactions:802.2 ; Chemical Products Generally:804

CHEMISTRY

Web of Science

1.School of Materials Science and Engineering, Harbin Institute of Technology, Nangang District, Harbin; 150001, China
2.Department of Materials Science and Engineering, Southern University of Science and Technology, Xili, Nanshan District, Shenzhen; 518055, China

Ning, Juewei,Tian, Yanqing. Development of a new simple mitochondria-targeted fluorescent K⁺ sensor and the application in high-throughput monitoring K⁺ fluxes[J]. SENSORS AND ACTUATORS B-CHEMICAL,2020,307.

Ning, Juewei,&Tian, Yanqing.(2020).Development of a new simple mitochondria-targeted fluorescent K⁺ sensor and the application in high-throughput monitoring K⁺ fluxes.SENSORS AND ACTUATORS B-CHEMICAL,307.

Ning, Juewei,et al."Development of a new simple mitochondria-targeted fluorescent K⁺ sensor and the application in high-throughput monitoring K⁺ fluxes".SENSORS AND ACTUATORS B-CHEMICAL 307(2020).