南方科技大学知识苑(SUSTech KC): Impact of Plasmid-Encoded H-NS-like Protein on blaNDM-1-Bearing IncX3 Plasmid in Escherichia coli

题名	Impact of Plasmid-Encoded H-NS-like Protein on blaNDM-1-Bearing IncX3 Plasmid in Escherichia coli
作者	Liu，Baomo 1; Shui，Lili 1,2; Zhou，Kai 3; Jiang，Ying 1; Li，Xiaoyu 1; Guan，Jing 1; Li，Qi 4; Zhuo，Chao 1
发表日期	2020-03-16
DOI	10.1093/infdis/jiz567
发表期刊	JOURNAL OF INFECTIOUS DISEASES 影响因子和分区
ISSN	0022-1899
EISSN	1537-6613
卷号	221 期号:2页码:S229-S236
摘要	BACKGROUND: This study was performed to assess the role of the histone-like nucleoid-structuring (H-NS)-like protein, carried by blaNDM-1-encoding IncX3-type plasmids, in the dissemination of IncX3 plasmids. METHODS: The blaNDM-1-encoding IncX3 plasmids were analyzed using southern blot, conjugation, and competition assays. Virulence was evaluated with a Galleria mellonella infection model. An hns-knockout IncX3 plasmid was also constructed to identify the functions of plasmid-borne H-NS-like protein in Escherichia coli. RESULTS: The assasys detected blaNDM-1-encoding IncX3-type plasmids with similar fingerprint patterns in all New Delhi metallo-β-lactamase (NDM) 1-producing carbapenem-resistant Enterobacteriaceae. The IncX3 plasmid conferred a fitness advantage to E. coli J53 but had no effect on host virulence. Moreover, the transconjugation frequency of the hns-null IncX3 plasmid pHN330-△hns was increased by 2.5-fold compared with the wild type. This was caused by up-regulation of conjugation-related plasmid-borne genes and the partition-related gene, in the J330-pHN330-△hns strain. In addition, decreased virulence was detected with this variant. CONCLUSIONS: Our results highlight the important role of IncX3 plasmids in the dissemination of blaNDM-1 in south China. Plasmid-encoded H-NS-like protein can inhibit plasmid conjugation, partition, and the expression of related genes, in addition to promoting virulence in the host.
关键词	bla NDM-1 E. coli dissemination H-NS-like protein IncX3 plasmid
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
重要成果	NI期刊
学校署名	其他
资助项目	National Natural Science Foundation of China[81772238][81861138056] ; Guangzhou Municipal Science and Technology Bureau[201607020044]
WOS研究方向	Immunology ; Infectious Diseases ; Microbiology
WOS类目	Immunology ; Infectious Diseases ; Microbiology
WOS记录号	WOS:000532694700012
出版者	OXFORD UNIV PRESS INC
ESI学科分类	IMMUNOLOGY
Scopus记录号	2-s2.0-85082000156
来源库	Scopus
引用统计	被引频次[WOS]：14
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/106343
专题	南方科技大学第一附属医院
作者单位	1.State Key Laboratory of Respiratory Disease,First Affiliated Hospital of Guangzhou Medical University,Guangzhou,China 2.Department of Pulmonary and Critical Care Medicine,First Affiliated Hospital of Chongqing Medical University,China 3.Shenzhen Institute of Respiratory Diseases,First Affiliated Hospital (Shenzhen People's Hospital),Southern University of Science and Technology,Second Clinical Medical College of Jinan University,Shenzhen,China 4.Pulmonary and Critical Care Medicine Center,Chinese PLA Respiratory Disease Institute,Xinqiao hospital,Army Medical University,Shapingba District,China
推荐引用方式 GB/T 7714	Liu，Baomo,Shui，Lili,Zhou，Kai,et al. Impact of Plasmid-Encoded H-NS-like Protein on blaNDM-1-Bearing IncX3 Plasmid in Escherichia coli[J]. JOURNAL OF INFECTIOUS DISEASES,2020,221(2):S229-S236.
APA	Liu，Baomo.,Shui，Lili.,Zhou，Kai.,Jiang，Ying.,Li，Xiaoyu.,...&Zhuo，Chao.(2020).Impact of Plasmid-Encoded H-NS-like Protein on blaNDM-1-Bearing IncX3 Plasmid in Escherichia coli.JOURNAL OF INFECTIOUS DISEASES,221(2),S229-S236.
MLA	Liu，Baomo,et al."Impact of Plasmid-Encoded H-NS-like Protein on blaNDM-1-Bearing IncX3 Plasmid in Escherichia coli".JOURNAL OF INFECTIOUS DISEASES 221.2(2020):S229-S236.