GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development

Zhang，Huihui1,2,3; Wang，Menglong1,2; Li，Yiqi1,2; Yan，Wei1,2; Chang，Zhenyi1,2; Ni，Haoling1; Chen，Zhufeng2; Wu，Jianxin1; Xu，Chunjue2; Deng，Xing Wang2,4; Tang，Xiaoyan1,2

2020

10.1111/jipb.12919

Journal of Integrative Plant Biology   影响因子和分区

1672-9072

1744-7909

Pollen exine contains complex biopolymers of aliphatic lipids and phenolics. Abnormal development of pollen exine often leads to plant sterility. Molecular mechanisms regulating exine formation have been studied extensively but remain ambiguous. Here we report the analyses of three GDSL esterase/lipase protein genes, OsGELP34, OsGELP110, and OsGELP115, for rice exine formation. OsGELP34 was identified by cloning of a male sterile mutant gene. OsGELP34 encodes an endoplasmic reticulum protein and was mainly expressed in anthers during pollen exine formation. osgelp34 mutant displayed abnormal exine and altered expression of a number of key genes required for pollen development. OsGELP110 was previously identified as a gene differentially expressed in meiotic anthers. OsGELP110 was most homologous to OsGELP115, and the two genes showed similar gene expression patterns. Both OsGELP110 and OsGELP115 proteins were localized in peroxisomes. Individual knockout of OsGELP110 and OsGELP115 did not affect the plant fertility, but double knockout of both genes altered the exine structure and rendered the plant male sterile. OsGELP34 is distant from OsGELP110 and OsGELP115 in sequence, and osgelp34 and osgelp110/osgelp115 mutants were different in anther morphology despite both were male sterile. These results suggested that OsGELP34 and OsGELP110/OsGELP115 catalyze different compounds for pollen exine development.

SCI

英语

其他

Major Program of Guangdong Basic and Applied Research[2019B030302006] ; National Natural Science Foundation of China[U1901203][31801344] ; Natural Science Foundation of Guangdong Province[2018B030308008] ; Shenzhen Commission on Innovation and Technology Programs[JCYJ20180507181837997] ; Guangzhou Science and Technology Innovation Commission[201804010034]

Biochemistry & Molecular Biology ; Plant Sciences

Biochemistry & Molecular Biology ; Plant Sciences

WOS:000563703400001

WILEY

2-s2.0-85082114070

Scopus

1.Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,School of Life Sciences,South China Normal University,Guangzhou,510631,China
2.Shenzhen Institute of Molecular Crop Design,Shenzhen,518107,China
3.School of Life Sciences,Capital Normal University,Beijing,100048,China
4.School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China

Zhang，Huihui,Wang，Menglong,Li，Yiqi,et al. GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development[J]. Journal of Integrative Plant Biology,2020,62(10):1574-1593.

Zhang，Huihui.,Wang，Menglong.,Li，Yiqi.,Yan，Wei.,Chang，Zhenyi.,...&Tang，Xiaoyan.(2020).GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development.Journal of Integrative Plant Biology,62(10),1574-1593.

Zhang，Huihui,et al."GDSL esterase/lipases OsGELP34 and OsGELP110/OsGELP115 are essential for rice pollen development".Journal of Integrative Plant Biology 62.10(2020):1574-1593.