Sublethal concentrations of triclosan elicited oxidative stress, DNA damage, and histological alterations in the liver and brain of adult zebrafish

Gyimah，Eric1,2; Dong，Xing1,2; Qiu，Wenhui3; Zhang，Zhen1,2; Xu，Hai1,2

2020

10.1007/s11356-020-08232-2

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH   影响因子和分区

0944-1344

1614-7499

Triclosan (TCS), an antimicrobial agent, has been a pollutant of increasing concern owing to its potential health risk on humans and aquatic animals. The present study seeks to test the hypothesis that TCS could alter the oxidative stress-related parameters in the brain and liver, as well as eliciting DNA damage in hepatocytes of adult zebrafish. On the basis of the 96 h LC (398.9 μg/L), adult zebrafish were separately exposed to 50, 100, and 150 μg/L TCS for 30 days. The brain and liver tissues from adult zebrafish were excised and assayed for a suite of antioxidant parameters and oxidative stress biomarkers including DNA damage in the liver. The induced effect by TCS on the activity of acetylcholinesterase (AChE) was also analyzed in the brain. Results showed a significant decrease in superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in the brain and liver of adult zebrafish. Also, the contents of the glutathione system (GSH and GSSH), as well as the activity of the glutathione reductase (GR), assayed in the liver, were reduced while the contents of malondialdehyde (MDA) were elevated in the liver. A comet assay revealed dose-dependent DNA damage in zebrafish hepatocytes. The 8-hydroxy-2′-deoxyguanosine (8-OHdG), MDA, and carbonyl protein contents in brain tissues significantly increased. Moreover, the AChE in the zebrafish brain was induced. Apparently, no obvious histological changes in brain tissues of zebrafish were observed compared with those of the control whereas atrophy and necrosis of hepatocytes and increased hepatic plate gap were observed in zebrafish hepatocytes after TCS exposure. The obtained results highlight that sublethal concentrations of TCS may be deleterious to the liver and brain of adult zebrafish upon subchronic exposure.

DNA damage Liver cells Oxidative stress Triclosan Zebrafish

SCI

英语

其他

National Natural Science Foundation of China[21577051] ; National Natural Science Foundation of China[21876067]

Environmental Sciences & Ecology

Environmental Sciences

WOS:000519502100010

SPRINGER HEIDELBERG

ENVIRONMENT/ECOLOGY

2-s2.0-85081919408

Scopus

1.Institute of Environmental Health and Ecological Security,School of Environment and Safety Engineering,Jiangsu University,Zhenjiang,212013,China
2.Jiangsu College of Water Treatment Technology and Material Collaborative Innovation Center,Suzhou,215009,China
3.School of Environmental Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China

Gyimah，Eric,Dong，Xing,Qiu，Wenhui,et al. Sublethal concentrations of triclosan elicited oxidative stress, DNA damage, and histological alterations in the liver and brain of adult zebrafish[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2020,27(14):17329-17338.

Gyimah，Eric,Dong，Xing,Qiu，Wenhui,Zhang，Zhen,&Xu，Hai.(2020).Sublethal concentrations of triclosan elicited oxidative stress, DNA damage, and histological alterations in the liver and brain of adult zebrafish.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,27(14),17329-17338.

Gyimah，Eric,et al."Sublethal concentrations of triclosan elicited oxidative stress, DNA damage, and histological alterations in the liver and brain of adult zebrafish".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 27.14(2020):17329-17338.