Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota

Wang，Yi1,2,3; Xu，Jiabao3; Kong，Lingchao1; Li，Bei4,5; Li，Hang5; Huang，Wei E.3; Zheng，Chunmiao2

2020

10.1111/1462-2920.14962

ENVIRONMENTAL MICROBIOLOGY   影响因子和分区

1462-2912

1462-2920

The antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) in human gut microbiota have significant impact on human health. While high throughput metagenomic sequencing reveals genotypes of microbial communities, the functionality, phenotype and heterogeneity of human gut microbiota are still elusive. In this study, we applied Raman microscopy and deuterium isotope probing (Raman–DIP) to detect metabolic active ARB (MA-ARB) in situ at the single-cell level in human gut microbiota from two healthy adults. We analysed the relative abundances of MA-ARB under different concentrations of amoxicillin, cephalexin, tetracycline, florfenicol and vancomycin. To establish the link between phenotypes and genotypes of the MA-ARB, Raman-activated cell sorting (RACS) was used to sort MA-ARB from human gut microbiota, and mini-metagenomic DNA of the sorted bacteria was amplified, sequenced and analysed. The sorted MA-ARB and their associated ARGs were identified. Our results suggest a strong relation between ARB in human gut microbiota and personal medical history. This study demonstrates that the toolkit of Raman–DIP, RACS and DNA sequencing can be useful to unravel both phenotypes and genotypes of ARB in human gut microbiota at the single-cell level.

SCI

英语

其他

Engineering and Physical Sciences Research Council[EP/M02833X/1][EP/M002403/1] ; National Natural Science Foundation of China[41931292][41890852] ; Natural Environment Research Council UK[NE/M002934/1]

Microbiology

Microbiology

WOS:000547588300014

WILEY

ENVIRONMENT/ECOLOGY

2-s2.0-85081736441

Scopus

1.School of Environment,Harbin Institute of Technology,Harbin,150090,China
2.Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control,School of Environmental Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
3.Department of Engineering Science,University of Oxford,Oxford,Parks Road,OX1 3PJ,United Kingdom
4.The State Key Lab of Applied Optics,Changchun Institute of Optics,Fine Mechanics and Physics,CAS,Changchun,130033,China
5.HOOKE Instruments Ltd.,Changchun,130033,China

Wang，Yi,Xu，Jiabao,Kong，Lingchao,et al. Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota[J]. ENVIRONMENTAL MICROBIOLOGY,2020,22(7):2613-2624.

Wang，Yi.,Xu，Jiabao.,Kong，Lingchao.,Li，Bei.,Li，Hang.,...&Zheng，Chunmiao.(2020).Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota.ENVIRONMENTAL MICROBIOLOGY,22(7),2613-2624.

Wang，Yi,et al."Raman-activated sorting of antibiotic-resistant bacteria in human gut microbiota".ENVIRONMENTAL MICROBIOLOGY 22.7(2020):2613-2624.