SMAD7 and SERPINE1 as novel dynamic network biomarkers detect and regulate the tipping point of TGF-beta induced EMT

Jiang，Zhonglin1; Lu，Lina1; Liu，Yuwei3,7; Zhang，Si1,5; Li，Shuxian1,5; Wang，Guanyu6; Wang，Peng2,5; Chen，Luonan1,4,5

2020

10.1016/j.scib.2020.01.013

Science Bulletin   影响因子和分区

2095-9273

2095-9281

Epithelial–mesenchymal transition (EMT) is a complex nonlinear biological process that plays essential roles in fundamental biological processes such as embryogenesis, wounding healing, tissue regeneration, and cancer metastasis. A hallmark of EMT is the switch-like behavior during state transition, which is characteristic of phase transitions. Hence, detecting the tipping point just before mesenchymal state transition is critical for understanding molecular mechanism of EMT. Through dynamic network biomarkers (DNB) model, a DNB group with 37 genes was identified which can provide the early-warning signals of EMT. Particularly, we found that two DNB genes, i.e. SMAD7 and SERPINE1 promoted EMT by switching their regulatory network which was further validated by biological experiments. Survival analyses revealed that SMAD7 and SERPINE1 as DNB genes further acted as prognostic biomarkers for lung adenocarcinoma.

Bistability Dynamic network biomarker Epithelial–mesenchymal transition Quantitative real-time PCR Tipping point

SCI ; EI

英语

通讯

National Key Research and Development Program of China[2017YFA0505500] ; National Natural Science Foundation of China[31930022][31771476][61773196] ; Shanghai Municipal Science and Technology Major Project[2017SHZDZX01] ; Key Project of Zhangjiang National Innovation Demonstration Zone Special Development Fund[ZJ2018ZD-013] ; Ministry of Science and Technology Project[2017YFC0907505] ; Guangdong Provincial Key Laboratory Funds[2017B030301018][2019B030301001] ; Shenzhen Research Funds[JCYJ20170307104535585][ZDSYS20140509142721429] ; Shenzhen Peacock Plan[KQTD2016053117035204]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000531830800013

ELSEVIER

20201108304953

Genes ; Diseases ; Polymerase chain reaction ; Tissue regeneration

Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Biochemistry:801.2

2-s2.0-85081564760

Scopus

1.Key Laboratory of Systems Biology,Center for Excellence in Molecular Cell Science,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai,200031,China
2.Bio-med Big Data Center,Key Laboratory of Computational Biology,CAS-MPG Partner Institute of Computational Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai,200031,China
3.Institute of Biomedical Sciences and School of Life Sciences,East China Normal University,Shanghai,200241,China
4.Center for Excellence in Animal Evolution and Genetics,Chinese Academy of Sciences,Kunming,650223,China
5.School of Life Science and Technology,ShanghaiTech University,Shanghai,201210,China
6.Department of Biology,Southern University of Science and Technology & Guangdong Provincial Key Laboratory of Cell Microenviroment and Disease Research & Guangdong Provincial Key Laboratory of Computational Science and Material Design,Shenzhen,518055,China
7.Laboratory of Systems Biology,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai,200031,China

Jiang，Zhonglin,Lu，Lina,Liu，Yuwei,et al. SMAD7 and SERPINE1 as novel dynamic network biomarkers detect and regulate the tipping point of TGF-beta induced EMT[J]. Science Bulletin,2020,65(10):842-853.

Jiang，Zhonglin.,Lu，Lina.,Liu，Yuwei.,Zhang，Si.,Li，Shuxian.,...&Chen，Luonan.(2020).SMAD7 and SERPINE1 as novel dynamic network biomarkers detect and regulate the tipping point of TGF-beta induced EMT.Science Bulletin,65(10),842-853.

Jiang，Zhonglin,et al."SMAD7 and SERPINE1 as novel dynamic network biomarkers detect and regulate the tipping point of TGF-beta induced EMT".Science Bulletin 65.10(2020):842-853.