Antitumor Effects and the Compatibility Mechanisms of Herb Pair Scleromitrion diffusum (Willd.) R. J. Wang-Sculellaria barbata D. Don

Lu, Li1; Zhan, Sheng1; Liu, Xiaohui1; Zhao, Xin2; Lin, Xiukun1; Xu, Huanli1

2020-03-20

10.3389/fphar.2020.00292

Frontiers in Pharmacology   影响因子和分区

1663-9812

Herb pair Scleromitrion diffusum (Willd.) R. J. Wang (HD) and Scutellaria barbata D. Don (SB) has been most frequently used for cancer treatment in traditional Chinese medicine. This study aimed to explore the in vitro and in vivo antitumor effects of HD-SB extract and to elucidate the underlying compatibility mechanisms. HD, SB, and HD-SB extracts were prepared, and the components were detected by ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method. The in vitro antitumor effects of various concentrations of these extract were detected on several tumor cell lines using MTS assay. The in vivo antitumor effects were evaluated in Panc28 cells-bearing nude mice model. The compatibility mechanisms of herb pair HD-SB were evaluated based on the systems pharmacology strategy and then validated by cellular experiments. HD-SB extract was demonstrated to inhibit the proliferation of the cancer cell lines dose dependently by MTS assay. In vivo antitumor effects of HD-SB were much more potent than either of the two single herbs in Panc28 xenograft mice model. A total 29 active ingredients involved in antitumor effects were selected from HD and SB, and the "herb-composition-target-disease" network was constructed. Then, 58 cancer-related targets and 66 KEGG pathways were identified, and PTGS2-, HSP90-, EGFR-, MMP2-, PPAR gamma-, and GSTP-mediated pathways were predicted to be the antitumor mechanisms of HD-SB. The cellular experiments showed that HD-SB significantly induced cancer cell apoptosis, decreased p-EGFR, HSP90 and bcl-2 expressions, and increased PPAR gamma, bax, cleaved caspase 3, cleaved PARP, p-AKT, and p-PI3K expressions compared with HD or SB treatment. Our study showed that HD-SB inhibited tumor growth both in vitro and in vivo, which might be related with apoptosis induction via the EGFR/PPAR gamma/PI3K/AKT pathway.

Scleromitrion diffusum (Willd ) R J Wang (HD) Scutellaria barbata D Don (SB) network pharmacology antitumor apoptosis

SCI

英语

其他

Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th 5-year Plan[CIT] ; Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th 5-year Plan[TCD201804086]

Pharmacology & Pharmacy

Pharmacology & Pharmacy

WOS:000523460200001

FRONTIERS MEDIA SA

Web of Science

1.Capital Med Univ, Dept Pharmacol, Sch Basic Med Sci, Beijing, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 2, Shenzhen, Peoples R China

Lu, Li,Zhan, Sheng,Liu, Xiaohui,et al. Antitumor Effects and the Compatibility Mechanisms of Herb Pair Scleromitrion diffusum (Willd.) R. J. Wang-Sculellaria barbata D. Don[J]. Frontiers in Pharmacology,2020,11.

Lu, Li,Zhan, Sheng,Liu, Xiaohui,Zhao, Xin,Lin, Xiukun,&Xu, Huanli.(2020).Antitumor Effects and the Compatibility Mechanisms of Herb Pair Scleromitrion diffusum (Willd.) R. J. Wang-Sculellaria barbata D. Don.Frontiers in Pharmacology,11.

Lu, Li,et al."Antitumor Effects and the Compatibility Mechanisms of Herb Pair Scleromitrion diffusum (Willd.) R. J. Wang-Sculellaria barbata D. Don".Frontiers in Pharmacology 11(2020).