南方科技大学知识苑(SUSTech KC): Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome

题名	Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome
作者	Dziekan, Jerzy Michal 1; Wirjanata, Grennady 1; Dai, Lingyun 1,5; Go, Ka Diam 1; Yu, Han 1; Lim, Yan Ting 2,3; Chen, Liyan 2,3; Wang, Loo Chien 2,3; Puspita, Brenda 1; Prabhu, Nayana 1; Sobota, Radoslaw M.2,3; Nordlund, Par 1,2,4; Bozdech, Zbynek 1
通讯作者	Sobota, Radoslaw M.; Nordlund, Par; Bozdech, Zbynek
发表日期	2020-04-27
DOI	10.1038/s41596-020-0310-z
发表期刊	Nature Protocols 影响因子和分区
ISSN	1754-2189
EISSN	1750-2799
卷号	15 期号:6页码:1881-1921
摘要	The authors demonstrate two variants of the cellular thermal shift assay (CETSA) protocol-the melt curve and the isothermal dose-response-for use on intact cell or lysate P. falciparum samples for identification of antimalarial drug targets.
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	Singapore Ministry of Education[MOE2015-T2-2-108]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000528992400001
出版者	NATURE PUBLISHING GROUP
来源库	Web of Science
引用统计	被引频次[WOS]：75
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/127153
专题	南方科技大学第一附属医院
作者单位	1.Nanyang Technol Univ, Sch Biol Sci, Singapore, Singapore 2.Agcy Sci Technol & Res, Inst Mol & Cell Biol, Singapore, Singapore 3.Agcy Sci Technol & Res, Inst Mol & Cell Biol, Funct Prote Lab, Singapore, Singapore 4.Karolinska Inst, Dept Pathol & Oncol, Stockholm, Sweden 5.Southern Univ Sci & Technol, Affiliated Hosp 1, Jinan Univ, Shenzhen Peoples Hosp,Clin Med Coll 2, Shenzhen, Peoples R China
推荐引用方式 GB/T 7714	Dziekan, Jerzy Michal,Wirjanata, Grennady,Dai, Lingyun,et al. Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome[J]. Nature Protocols,2020,15(6):1881-1921.
APA	Dziekan, Jerzy Michal.,Wirjanata, Grennady.,Dai, Lingyun.,Go, Ka Diam.,Yu, Han.,...&Bozdech, Zbynek.(2020).Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome.Nature Protocols,15(6),1881-1921.
MLA	Dziekan, Jerzy Michal,et al."Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome".Nature Protocols 15.6(2020):1881-1921.