Cutting Edge: Characterization of Human Tissue-Resident Memory T Cells at Different Infection Sites in Patients with Tuberculosis

Yang, Qianting1,2; Zhang, Mingxia2; Chen, Qi2; Chen, Weixin2; Wei, Cailin2; Qiao, Kun2; Ye, Taosheng2; Deng, Guofang2; Li, Jin3; Zhu, Jialou4; Cai, Yi4; Chen, Xinchun4; Ma, Li1

2020-05

10.4049/jimmunol.1901326

JOURNAL OF IMMUNOLOGY   影响因子和分区

0022-1767

1550-6606

Tissue-resident memory T cells (TRMs) have a key role in mediating the host defense against tuberculosis (TB) in mice, but their human counterparts have not been well characterized. In this article, we recruited patients with TB and determined TRM frequency, trafficking, activation marker expression, and cytokine production by flow or mass cytometry at different infection sites, including peripheral blood, pleural fluid, bronchoalveolar lavage fluid, and lung. We found a high frequency of TRMs at all infection sites apart from the peripheral blood. These TRMs exhibited a memory phenotype, were highly activated (based on CD38 and HLA-DR expression), and expressed high levels of trafficking (CCR5 and CXCR6) and exhaustion (PD-1) markers. When stimulated with Mycobacterium tuberculosis, TRMs secreted cytokines, including IFN-gamma, TNF-alpha, and IL-2, and exhibited a multifunctional phenotype. TRMs limited intracellular M. tuberculosis replication in macrophages. These data inform our current understanding of immunosurveillance at different infection sites in patients with TB.

SCI

英语

其他

Science and Technology Project of Shenzhen[JCYJ20160427184123851] ; Science and Technology Project of Shenzhen[SZSM201412001]

Immunology

Immunology

WOS:000528255900002

AMER ASSOC IMMUNOLOGISTS

IMMUNOLOGY

Web of Science

1.Southern Med Univ, Sch Lab Med & Biotechnol, Inst Mol Immunol, 1838th Guangzhou Ave North, Guangzhou 510515, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Guangdong Key Lab Diag & Treatment Emerging Infec, Shenzhen 518112, Peoples R China
3.Sun Yat Sen Univ, Affiliated Hosp 7, Dept Pulm & Crit Care Med, Shenzhen 518107, Peoples R China
4.Shenzhen Univ, Dept Pathogen Biol, Guangdong Key Lab Reg Immun & Dis, Sch Med, Shenzhen 518060, Peoples R China

Yang, Qianting,Zhang, Mingxia,Chen, Qi,et al. Cutting Edge: Characterization of Human Tissue-Resident Memory T Cells at Different Infection Sites in Patients with Tuberculosis[J]. JOURNAL OF IMMUNOLOGY,2020,204(9):2331-2336.

Yang, Qianting.,Zhang, Mingxia.,Chen, Qi.,Chen, Weixin.,Wei, Cailin.,...&Ma, Li.(2020).Cutting Edge: Characterization of Human Tissue-Resident Memory T Cells at Different Infection Sites in Patients with Tuberculosis.JOURNAL OF IMMUNOLOGY,204(9),2331-2336.

Yang, Qianting,et al."Cutting Edge: Characterization of Human Tissue-Resident Memory T Cells at Different Infection Sites in Patients with Tuberculosis".JOURNAL OF IMMUNOLOGY 204.9(2020):2331-2336.