Synthetic biohybrid peptidoglycan oligomers enable pan-bacteria-specific labeling and imaging: in vitro and in vivo

He, Jing-Xi1,2; Hoang, Kim Le Mai1; Kho, Shu Hui1,3; Guo, Zhong1; Zhong, Wenbin2; Thappeta, Kishore Reddy Venkata2; Zamudio-Vazquez, Rubi2; Hoo, Sin Ni1; Xiong, Qirong2; Duan, Hongwei2; Yang, Liang4; Chan-Park, Mary B.2; Liu, Xue-Wei1

2020-03-28

10.1039/c9sc06345e

Chemical Science   影响因子和分区

2041-6520

2041-6539

Peptidoglycan is the core component of the bacterial cell wall, which makes it an attractive target for the development of bacterial targeting agents. Intercepting its enzymatic assembly with synthetic substrates allows for labeling and engineering of live bacterial cells. Over the past two decades, small-molecule-based labeling agents, such as antibiotics, d-amino acids or monosaccharides have been developed for probing biological processes in bacteria. Herein, peptidoglycan oligomers, substrates for transglycosylation, are prepared for the first time using a top-down approach, which starts from chitosan as a cheap feedstock. A high efficiency of labeling has been observed in all bacterial strains tested using micromolar substrates. In contrast, uptake into mammalian cells was barely observable. Additional mechanistic studies support a hypothesis of bacteria-specific metabolic labeling rather than non-specific binding to the bacterial surface. Eventually, its practicality in bacterial targeting capability is demonstrated in resistant strain detection and in vivo infection models.

SCI ; EI

英语

NI论文

其他

Nanyang Technological University[RG120/18]

Chemistry

Chemistry, Multidisciplinary

WOS:000528663000003

ROYAL SOC CHEMISTRY

20201508384716

Oligomers ; Cell engineering ; Cell membranes ; Mammals

Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Organic Polymers:815.1.1

Web of Science

1.Nanyang Technol Univ, Sch Phys & Math Sci, 21 Nanyang Link, Singapore 637371, Singapore
2.Nanyang Technol Univ, Sch Chem & Biomed Engn, 62 Nanyang Dr, Singapore 637459, Singapore
3.Nanyang Technol Univ, NTU Inst Hlth Technol, Singapore, Singapore
4.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China

He, Jing-Xi,Hoang, Kim Le Mai,Kho, Shu Hui,et al. Synthetic biohybrid peptidoglycan oligomers enable pan-bacteria-specific labeling and imaging: in vitro and in vivo[J]. Chemical Science,2020,11(12):3171-3179.

He, Jing-Xi.,Hoang, Kim Le Mai.,Kho, Shu Hui.,Guo, Zhong.,Zhong, Wenbin.,...&Liu, Xue-Wei.(2020).Synthetic biohybrid peptidoglycan oligomers enable pan-bacteria-specific labeling and imaging: in vitro and in vivo.Chemical Science,11(12),3171-3179.

He, Jing-Xi,et al."Synthetic biohybrid peptidoglycan oligomers enable pan-bacteria-specific labeling and imaging: in vitro and in vivo".Chemical Science 11.12(2020):3171-3179.