Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging

Liu, Zhihe1,2; Liu, Jie1,3; Zhang, Zhe2; Sun, Zezhou2; Shao, Xuyang1; Guo, Jintong1; Xi, Lei1; Yuan, Zhen4; Zhang, Xuanjun4; Chiu, Daniel T.5; Wu, Changfeng1

2020-04-14

10.1039/d0nr00347f

Nanoscale   影响因子和分区

2040-3364

2040-3372

Super-resolution optical fluctuation imaging (SOFI) produces fast, background-free, super-resolved images by analyzing the temporal fluorescence fluctuations of independent emitters. With sufficient brightness and fluctuations, a higher order of image processing affords a higher resolution and in principle the resolution enhancement is unbounded. However, it is practically challenging to find suitable probes for high-order SOFI. Herein, we report two types of BODIPY-based polymer dots (Pdots) with narrow-band emissions, pronounced fluctuations, and prominent photostability, thus enabling high-order, dual-color SOFI nanoscopy. Single-particle and subcellular SOFI analysis reveals the superior performance of the BODIPY Pdots as compared to conventional streptavidin-conjugated Alexa dyes. In contrast with wide-field images, the spatial resolution (similar to 57 nm) was enhanced by similar to 6.0-fold in 8(th)-order single-particle SOFI nanoscopy. A spatial resolution (61 nm) was obtained for single microtubules labeled by the BODIPY Pdots, while the majority of the subcellular structures were lost for those labeled by streptavidin-Alexa dyes in 8(th)-order SOFI. This work indicates the unprecedented performance of Pdot probes for multi-color subcellular SOFI applications.

SCI ; EI

英语

第一 ; 通讯

Shenzhen Science and Technology Innovation Commission[KQTD20170810111314625] ; National Natural Science Foundation of China[81771930]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied

WOS:000529531500008

ROYAL SOC CHEMISTRY

20201708510452

Image resolution ; Optical data processing ; Probes ; Fluorescence ; Color ; Image enhancement

Data Processing and Image Processing:723.2 ; Light/Optics:741.1 ; Organic Compounds:804.1

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 510855, Guangdong, Peoples R China
2.Jilin Univ, State Key Lab Integrated Optoelect, Coll Elect Sci & Engn, Changchun 130012, Jilin, Peoples R China
3.Hong Kong Baptist Univ, Dept Biol, Hong Kong, Peoples R China
4.Univ Macau, Fac Hlth Sci, Taipa 999078, Macau, Peoples R China
5.Univ Washington, Dept Chem, Seattle, WA 98195 USA

Liu, Zhihe,Liu, Jie,Zhang, Zhe,et al. Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging[J]. Nanoscale,2020,12(14):7522-7526.

Liu, Zhihe.,Liu, Jie.,Zhang, Zhe.,Sun, Zezhou.,Shao, Xuyang.,...&Wu, Changfeng.(2020).Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging.Nanoscale,12(14),7522-7526.

Liu, Zhihe,et al."Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging".Nanoscale 12.14(2020):7522-7526.