南方科技大学知识苑(SUSTech KC): Visualization of Ligand-Bound Ectodomain Assembly in the Full-Length Human IGF-1 Receptor by Cryo-EM Single-Particle Analysis

题名	Visualization of Ligand-Bound Ectodomain Assembly in the Full-Length Human IGF-1 Receptor by Cryo-EM Single-Particle Analysis
作者	Zhang, Xi1,2 ; Yu, Daqi 3; Sun, Jingchuan 4; Wu, Yujie 4; Gong, Junyuan2 ; Li, Xuemei 5; Liu, Li2 ; Liu, Shan2 ; Liu, Jianbo 4; Wu, Yulan2 ; Li, Dongyang2 ; Ma, Yinping 6; Han, Xu 3; Zhu, Yanan 3; Wu, Zhaolong 3; Wang, Yihua 3; Ouyang, Qi 3,7,8; Wang, Tao2,4,9
通讯作者	Wang, Tao
发表日期	2020-05-05
DOI	10.1016/j.str.2020.03.007
发表期刊	STRUCTURE 影响因子和分区
ISSN	0969-2126
EISSN	1878-4186
卷号	28 期号:5页码:555-+
摘要	Tyrosine kinase receptor of insulin-like growth factor 1 receptor (IGF-1R) and insulin receptor (IR) bind to hormones, such as insulin, IGF-1, and IGF-2, and transduces the signals across the cell membrane, However, the complete structure of the receptor and the signal transduction mechanism remains unclear. Here, we report the cryo-EM structure of the ligand-bound ectodomain in the full-length human IGF-1 R. We reconstructed the IGF-1 R/insulin complex at 4.7 angstrom and the IGF-1 R/IGF-1 complex at 7.7 angstrom. Our structures reveal that only one insulin or one IGF-1 molecule binds to and activates the full-length human IGF-1R receptor.
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	National Key R&D Program of China[2018YFA0507103] ; National Major Scientific and Technological Special Project[2018ZX09711003-003-004][2019ZX09721001-004-007] ; National Natural Science Foundation of China[31870719][11774011] ; Shenzhen San-Ming Project[SZSM201809085] ; Shenzhen STIC[JCYJ20170307-110657570][JCYC20170817-110434640]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号	WOS:000531055000009
出版者	CELL PRESS
ESI学科分类	BIOLOGY & BIOCHEMISTRY
来源库	Web of Science
引用统计	被引频次[WOS]：21
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/137771
专题	生命科学学院_生物系
作者单位	1.Harbin Inst Technol, Sch Life Sci & Technol, Harbin 150001, Peoples R China 2.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Peoples R China 3.Peking Univ, Sch Phys, State Key Lab Artificial Microstruct & Mesoscop P, Beijing 100871, Peoples R China 4.Peking Univ, Inst Syst Biol, Shenzhen Bay Lab, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China 5.Peking Univ, Sch Phys, Electron Microscopy Lab, Beijing 100871, Peoples R China 6.Peking Univ, Comp Ctr, Beijing 100871, Peoples R China 7.Peking Univ, Peking Tsinghua Ctr Life Sci, Sch Phys, Beijing 100871, Peoples R China 8.Peking Univ, Ctr Quantitat Biol, Beijing 100871, Peoples R China 9.Longhua Ctr Dis Control & Prevent, Joint Lab Infect Dis Prevent & Control, Hygien Sect, Shenzhen 518109, Peoples R China
第一作者单位	生物系
通讯作者单位	生物系
推荐引用方式 GB/T 7714	Zhang, Xi,Yu, Daqi,Sun, Jingchuan,et al. Visualization of Ligand-Bound Ectodomain Assembly in the Full-Length Human IGF-1 Receptor by Cryo-EM Single-Particle Analysis[J]. STRUCTURE,2020,28(5):555-+.
APA	Zhang, Xi.,Yu, Daqi.,Sun, Jingchuan.,Wu, Yujie.,Gong, Junyuan.,...&Wang, Tao.(2020).Visualization of Ligand-Bound Ectodomain Assembly in the Full-Length Human IGF-1 Receptor by Cryo-EM Single-Particle Analysis.STRUCTURE,28(5),555-+.
MLA	Zhang, Xi,et al."Visualization of Ligand-Bound Ectodomain Assembly in the Full-Length Human IGF-1 Receptor by Cryo-EM Single-Particle Analysis".STRUCTURE 28.5(2020):555-+.