Ultrahigh-sensitivity label-free optical fiber biosensor based on a tapered singlemode- no core-singlemode coupler for Staphylococcus aureus detection

Chen，Ling1,2; Leng，Yuan Kui3; Liu，Bin1; Liu，Juan1; Wan，Sheng Peng1; Wu，Tao1; Yuan，Jinhui4; Shao，Liyang5; Gu，Guoqiang5; Fu，Yong Qing2; Xu，Hengyi3; Xiong，Yonghua3; He，Xing Dao1; Wu，Qiang1,2

2020-10-01

10.1016/j.snb.2020.128283

SENSORS AND ACTUATORS B-CHEMICAL   影响因子和分区

0925-4005

0925-4005

An ultra-high sensitivity label-free optical fiber biosensor for inactivated Staphylococcus aureus (S. aureus) detection is proposed and investigated in this study, with additional advantages of robust and stability compared to traditional tapered fiber structure. The proposed fiber biosensor is based on a tapered singlemode- no core-singlemode fiber coupler (SNSFC) structure, where the no core fiber was tapered to small diameter (taper-waist diameter of about 10 μm) and functionalized with the pig immunoglobulin G (IgG) antibody for detection of S. aureus. The measured maximum wavelength shift of the sensor for an S. aureus concentration of 7 × 10 CFU/mL (colony forming unit per milliliter) is 2.04 nm, which is equivalent to a limit of detection (LOD) of 3.1 CFU/mL (a highest LOD reported so far for optical fiber biosensors), considering the maximum wavelength variation of the sensor in phosphate buffered saline (PBS) is ±0.03 nm over 40 min, where 3 times of maximum wavelength variation (3 × 0.03 = 0.09 nm) is defined as measurement limit. The response time of the developed fiber sensor is less than 30 min. The ultra-sensitive biosensor has potential to be widely applied to various areas such as disease, medical diagnostics and food safety inspection.

Biosensor Foodborne pathogens Optical fiber sensor Staphylococcus aureus (S. aureus)

SCI ; EI

英语

其他

Nanchang Hangkong University graduate student innovation special fund project[YC2019049] ; Natural Science Foundation of Jiangxi Province[20192ACB20031][20192ACBL21051] ; State Key Laboratory of Advanced Optical Communication Systems and Networks, Shanghai Jiao Tong University, China[2018G2KF03003] ; National Natural Science Foundation of China[61665007][61465009] ; Major Academic and Technical Leaders Funding Program of Jiangxi,China[20172BCB22012]

Chemistry ; Electrochemistry ; Instruments & Instrumentation

Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation

WOS:000558700900005

ELSEVIER SCIENCE SA

20202308784774

Biosensors ; Chemical detection ; Mammals ; Optical fibers ; Antibodies ; Diagnosis ; Food safety

Medicine and Pharmacology:461.6 ; Immunology:461.9.1 ; Fiber Optics:741.1.2 ; Chemistry:801 ; Food Products:822.3

CHEMISTRY

2-s2.0-85085700273

Scopus

1.Key Laboratory of Nondestructive Test (Ministry of Education),Nanchang Hangkong University,Nanchang,330063,China
2.Faculty of Engineering and Environment,Northumbria University,Newcastle Upon Tyne,NE1 8ST,United Kingdom
3.State Key Lab Food Sci & Technol,Nanchang University,Nanchang,China
4.Research Center for Convergence Networks and Ubiquitous Services,University of Science & Technology Beijing,Beijing,100083,China
5.Department of Electrical and Electronic Engineering,Southern University of Science and Technology of China,Shenzhen,China

Chen，Ling,Leng，Yuan Kui,Liu，Bin,et al. Ultrahigh-sensitivity label-free optical fiber biosensor based on a tapered singlemode- no core-singlemode coupler for Staphylococcus aureus detection[J]. SENSORS AND ACTUATORS B-CHEMICAL,2020,320.

Chen，Ling.,Leng，Yuan Kui.,Liu，Bin.,Liu，Juan.,Wan，Sheng Peng.,...&Wu，Qiang.(2020).Ultrahigh-sensitivity label-free optical fiber biosensor based on a tapered singlemode- no core-singlemode coupler for Staphylococcus aureus detection.SENSORS AND ACTUATORS B-CHEMICAL,320.

Chen，Ling,et al."Ultrahigh-sensitivity label-free optical fiber biosensor based on a tapered singlemode- no core-singlemode coupler for Staphylococcus aureus detection".SENSORS AND ACTUATORS B-CHEMICAL 320(2020).