Molecular genetic analysis of an XDR Pseudomonas aeruginosa ST664 clone carrying multiple conjugal plasmids

Li，Zhenpeng1; Cai，Zhao2; Cai，Zeqiong1; Zhang，Yanhong3; Fu，Tongtong1; Jin，Yongxin1; Cheng，Zhihui1; Jin，Shouguang4; Wu，Weihui1; Yang，Liang5; Bai，Fang1

2020-06-01

10.1093/jac/dkaa063

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY   影响因子和分区

0305-7453

1460-2091

OBJECTIVES: A group of ST664 XDR Pseudomonas aeruginosa strains have been isolated from a burn clinic. Here we decipher their resistomes and likely mechanisms of resistance acquisition. METHODS: The complete nucleotide sequences of representative isolates were determined, by PacBio and Illumina MiSeq sequencing, and analysed for antimicrobial resistance (AMR) genes as well as sequence variations. S1-PFGE was used to determine the sizes and numbers of plasmids harboured by the isolates. Purified plasmid DNA was further sequenced by PacBio technology, closed manually and annotated by RAST. The mobility of plasmids was determined by conjugation assays. RESULTS: The XDR P. aeruginosa ST664 clone carries 11 AMR genes, including a blaKPC-2 gene that confers resistance to carbapenems. Most of the ST664 isolates carry three coexisting plasmids. blaKPC-2 and a cluster of three AMR genes (aadB-cmlA1-sul1) are encoded on a 475 kb megaplasmid pNK546a, which codes for an IncP-3-like replication and partitioning mechanism, but has lost the conjugative transfer system. Interestingly, however, pNK546a is mobilizable and can be transferred to P. aeruginosa PAO1 with the help of a co-residing IncP-7 conjugative plasmid. The blaKPC-2 gene is carried by an IS6100-ISKpn27-blaKPC-2-ΔISKpn6-Tn1403 mobile element, which might be brought into the ST664 clone by another co-resident IncP-1α plasmid, which is inclined to be lost. Moreover, pNK546a harbours multiple heavy metal (mercury, tellurite and silver) resistance modules. CONCLUSIONS: To the best of our knowledge, pNK546a is the first fully sequenced blaKPC-2-carrying megaplasmid from P. aeruginosa. These results give new insights into bacterial adaptation and evolution during nosocomial infections.

SCI

英语

其他

National Key Research and Development Project of China[2017YFE0125600] ; Natural Science Foundation of China[31870130][31970680][31970179] ; Tianjin Municipal Science and Technology Commission of China[17JCQNJC09200][19JCYBJC24700] ; Fundamental Research Funds for the Central Universities of China, Nankai University[63191117] ; Southern University of Science and Technology (SUSTech)[Y01416206] ; Chinese Ministry of Education Changjiang Scholar Program[Q2018288] ; Shenzhen Peacock Plan Research Grant of China[20190128110B]

Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy

Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy

WOS:000567185800012

OXFORD UNIV PRESS

PHARMACOLOGY & TOXICOLOGY

2-s2.0-85085264782

Scopus

1.State Key Laboratory of Medicinal Chemical Biology,Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education,College of Life Sciences,Nankai University,China
2.Singapore Centre for Environmental Life Sciences Engineering,Nanyang Technological University,60 Nanyang Drive,Singapore
3.Affiliated Hospital of Nankai University,China
4.Department of Molecular Genetics and Microbiology,University of Florida,FL,Gainesville,United States
5.School of Medicine,Southern University of Science and Technology (SUSTech),Shenzhen,China

Li，Zhenpeng,Cai，Zhao,Cai，Zeqiong,et al. Molecular genetic analysis of an XDR Pseudomonas aeruginosa ST664 clone carrying multiple conjugal plasmids[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY,2020,75(6):1443-1452.

Li，Zhenpeng.,Cai，Zhao.,Cai，Zeqiong.,Zhang，Yanhong.,Fu，Tongtong.,...&Bai，Fang.(2020).Molecular genetic analysis of an XDR Pseudomonas aeruginosa ST664 clone carrying multiple conjugal plasmids.JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY,75(6),1443-1452.

Li，Zhenpeng,et al."Molecular genetic analysis of an XDR Pseudomonas aeruginosa ST664 clone carrying multiple conjugal plasmids".JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 75.6(2020):1443-1452.