Vitellogenin Receptor (VgR) Mediates Oocyte Maturation and Ovarian Development in the Pacific White Shrimp (Litopenaeus vannamei)

Ruan，Yao1,2; Wong，Nai Kei3; Zhang，Xin1,2; Zhu，Chunhua4; Wu，Xiaofen1,2; Ren，Chunhua1,5; Luo，Peng1,5; Jiang，Xiao1,5; Ji，Jiatai1,6; Wu，Xugan7; Hu，Chaoqun1,5; Chen，Ting1,5

2020-05-15

10.3389/fphys.2020.00485

Frontiers in Physiology   影响因子和分区

1664-042X

1664-042X

Oocyte maturation and ovarian development are sequentially coordinated events critical to reproduction. In the ovaries of adult oviparous animals such as birds, bony fish, insects, and crustaceans, vitellogenin receptor (VgR) is a plasma membrane receptor that specifically mediates vitellogenin (Vg) transport into oocytes. Accumulation of Vg drives sexual maturation of the female crustaceans by acting as a pivotal regulator of nutritional accumulation within oocytes, a process known as vitellogenesis. However, the mechanisms by which VgR mediates vitellogenesis are still not fully understood. In this study, we first identified a unique VgR (Lv-VgR) and characterized its genomic organization and protein structural domains in Litopenaeus vannamei, a predominant cultured shrimp species worldwide. This newly identified Lv-VgR phylogenetically forms a group with VgRs from other crustacean species within the arthropod cluster. Duplicated LBD/EGFD regions are found exclusively among arthropod VgRs but not in paralogs from vertebrates and nematodes. In terms of expression patterns, Lv-VgR transcripts are specifically expressed in ovaries of female shrimps, which increases progressively during ovarian development, and rapidly declines toward embryonic development. The cellular and subcellular locations were For analyzed by in situ hybridization and immunofluorescence, respectively. The Lv-VgR mRNA was found to be expressed in the oocytes of ovaries, and Lv-VgR protein was found to localize in the cell membrane of maturing oocytes while accumulation of the ligand Vg protein assumed an even cytoplasmic distribution. Silencing of VgR transcript expression by RNAi was effective for stunting ovarian development. This present study has thus provided new insights into the regulatory roles of VgR in crustacean ovarian development.

crustacean mRNA expression oocyte maturation ovarian development phylogenetic analysis vitellogenesis vitellogenin receptor

SCI

英语

其他

Science & Technology Project of Guangzhou[201904020009] ; Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams[2019KJ149] ; Guangdong Province Program[2017B030314052][2017A030310429][2016A020210062] ; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences[ISEE2018PY03] ; Shanghai talents development fund for young scientists[2018100]

Physiology

Physiology

WOS:000538425300001

FRONTIERS MEDIA SA

2-s2.0-85085592077

Scopus

1.CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB),Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB),South China Sea Institute,of Oceanology,Chinese Academy of Sciences,Guangzhou,China
2.University of Chinese Academy of Sciences,Beijing,China
3.National Clinical Research Center for Infectious Diseases,Shenzhen Third People’s Hospital,The Second Hospital,Southern University of Science and Technology,Shenzhen,China
4.College of Fisheries,Guangdong Ocean University,Zhanjiang,China
5.Institution of South China Sea Ecology and Environmental Engineering (ISEE),Chinese Academy of Sciences,Guangzhou,China
6.Guangdong Haimao Investment Co.,Ltd,Zhanjiang,China
7.Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture,Shanghai Ocean University,Shanghai,China

Ruan，Yao,Wong，Nai Kei,Zhang，Xin,et al. Vitellogenin Receptor (VgR) Mediates Oocyte Maturation and Ovarian Development in the Pacific White Shrimp (Litopenaeus vannamei)[J]. Frontiers in Physiology,2020,11.

Ruan，Yao.,Wong，Nai Kei.,Zhang，Xin.,Zhu，Chunhua.,Wu，Xiaofen.,...&Chen，Ting.(2020).Vitellogenin Receptor (VgR) Mediates Oocyte Maturation and Ovarian Development in the Pacific White Shrimp (Litopenaeus vannamei).Frontiers in Physiology,11.

Ruan，Yao,et al."Vitellogenin Receptor (VgR) Mediates Oocyte Maturation and Ovarian Development in the Pacific White Shrimp (Litopenaeus vannamei)".Frontiers in Physiology 11(2020).