Phagocyte Transcriptomic Analysis Reveals Focal Adhesion Kinase (FAK) and Heparan Sulfate Proteoglycans (HSPGs) as Major Regulators in Anti-bacterial Defense of Crassostrea hongkongensis

Lin，Yue1,2; Mao，Fan1,3,4; Wong，Nai Kei5; Zhang，Xiangyu1,2; Liu，Kunna1,2; Huang，Minwei1,3,4; Ma，Haitao1,3,4; Xiang，Zhiming1,3,4; Li，Jun1,3,4; Xiao，Shu1,3,4; Zhang，Yang1,3,4; Yu，Ziniu1,3,4

2020-03-20

10.3389/fimmu.2020.00416

Frontiers in Immunology   影响因子和分区

1664-3224

1664-3224

Invertebrates generally lack adaptive immunity and compensate for this with highly efficient innate immune machineries such as phagocytosis by hemocytes to eradicate invading pathogens. However, how extrinsically cued hemocytes marshal internal signals to accomplish phagocytosis is not yet fully understood. To this end, we established a facile magnetic cell sorting method to enrich professional phagocytes from hemocytes of the Hong Kong oyster (Crassostrea hongkongensis), an ecologically and commercially valuable marine invertebrate. Transcriptomic analysis on presorted cells shows that phagocytes maintain a remarkable array of differentially expressed genes that distinguish them from non-phagocytes, including 352 significantly upregulated genes and 479 downregulated genes. Pathway annotations reveal that focal adhesion and extracellular matrix–receptor interactions were the most conspicuously enriched pathways in phagocytes. Phagocytosis rate dramatically declined in the presence of an FAK inhibitor, confirming importance of the focal adhesion pathway in regulating phagocytosis. In addition, we also found that heparan sulfate proteoglycan (HSPG) families were lineage-specifically expanded in C. hongkongensis and abundantly expressed in phagocytes. Efficiency of phagocytosis and hemocytes aggregation was markedly reduced upon blockage of endogenous synthesis of HSPGs, thus implicating these proteins as key surface receptors in pathogen recognition and initiation of phagocytosis.

Crassostrea hongkongensis focal adhesion kinase (FAK) heparan sulfate proteoglycans (HSPGs) phagocytes transcriptome

SCI

英语

其他

WOS:000525534600001

2-s2.0-85083052677

Scopus

1.CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,Guangdong Provincial Key Laboratory of Applied Marine Biology,South China Sea Institute of Oceanology,Chinese Academy of Science,Guangzhou,China
2.University of Chinese Academy of Sciences,Beijing,China
3.Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE),Chinese Academy of Sciences,Guangzhou,China
4.Southern Marine Science and Engineering Guangdong Laboratory,Guangzhou,China
5.National Clinical Research Center for Infectious Diseases,Shenzhen Third People's Hospital,The Second Hospital,Southern University of Science and Technology,Shenzhen,China

Lin，Yue,Mao，Fan,Wong，Nai Kei,et al. Phagocyte Transcriptomic Analysis Reveals Focal Adhesion Kinase (FAK) and Heparan Sulfate Proteoglycans (HSPGs) as Major Regulators in Anti-bacterial Defense of Crassostrea hongkongensis[J]. Frontiers in Immunology,2020,11.

Lin，Yue.,Mao，Fan.,Wong，Nai Kei.,Zhang，Xiangyu.,Liu，Kunna.,...&Yu，Ziniu.(2020).Phagocyte Transcriptomic Analysis Reveals Focal Adhesion Kinase (FAK) and Heparan Sulfate Proteoglycans (HSPGs) as Major Regulators in Anti-bacterial Defense of Crassostrea hongkongensis.Frontiers in Immunology,11.

Lin，Yue,et al."Phagocyte Transcriptomic Analysis Reveals Focal Adhesion Kinase (FAK) and Heparan Sulfate Proteoglycans (HSPGs) as Major Regulators in Anti-bacterial Defense of Crassostrea hongkongensis".Frontiers in Immunology 11(2020).