Metal-to-Ligand Charge Transfer Chirality Sensing of d-Glucose Assisted with GOX-Based Enzymatic Reaction

Chiral transition metal oxides nanoparticles (NPs) with tunable optical properties are widely accepted as promising toolbox for chiral recognition, stereoselective synthesis, and chiroptical devices. Herein, chirality-based strategy is presented for discrimination of d-glucose from its enantiomer through a cooperative synergy between chiral cysteine capped MoO NPs and the glucose oxidase nanosystem. The valence-state-dependent chirality induced by metal-ligand charge transfer effect is found to be ultrasensitive to its redox environment such as the presence of hydrogen peroxide, which is a key indicator of the stereoselective enzymatic reaction between glucose oxidase and d-glucose. With this know-how, glucose enantiomers can be precisely quantified with a limit of detection of 0.446 µm. Such a chiral bio-nanosystem would be an ideal platform for a rational design of new types of biosensors, photocatalysts, and chirality-based nanodevices.