Temporal Modulations of NODAL, BMP, and WNT Signals Guide the Spatial Patterning in Self-Organized Human Ectoderm Tissues

Xie，Tianfa1; Kang，Jiming2; Pak，Chang Hui3; Yuan，Hongyan2; Sun，Yubing1,4

2020

10.1016/j.matt.2020.04.012

Matter   影响因子和分区

2590-2393

2590-2385

Developmental biology studies using model organisms suggest that the emergence of spatial patterning in the ectoderm is mediated by the morphogen gradients. However, it is still unclear whether the morphogen gradient is necessary and dominates the cell spatial patterning, particularly in human genetic background. Here, we demonstrate that human pluripotent stem cells can self-organize to concentric rings of all major cell types in ectoderm when cultured on micropatterned surfaces in a chemically defined condition. We reveal that modulating the dynamics of NODAL, BMP, and WNT signals is sufficient to control the spatial order of different cell types. Our mathematical model suggests that changes in wavelength and phase of signaling patterns formed via reaction-diffusion may be the mechanism by which temporal information is translated into spatial information. Together, our work demonstrates that in vitro human ectoderm microtissues have great potential in understanding the mechanisms of early-stage human development.

BMP ectoderm human embryonic stem cells MAP3: Understanding mechanotransduction neural crest organoids placode reaction-diffusion WNT

ESCI ; EI ; SCI

英语

其他

National Science Foundation[CMMI 1662835][CMMI 1846866]

Materials Science

Materials Science, Multidisciplinary

WOS:000539230500005

ELSEVIER

20202108685377

Diffusion in liquids

Biological Materials and Tissue Engineering:461.2

2-s2.0-85084822808

Scopus

1.Department of Mechanical and Industrial Engineering,University of Massachusetts,Amherst,01003,United States
2.Department of Mechanics and Aerospace Engineering,Southern University of Science and Technology,Shenzhen,518055,China
3.Department of Biochemistry and Molecular Biology,University of Massachusetts,Amherst,01003,United States
4.Department of Chemical Engineering,University of Massachusetts,Amherst,01003,United States

Xie，Tianfa,Kang，Jiming,Pak，Chang Hui,et al. Temporal Modulations of NODAL, BMP, and WNT Signals Guide the Spatial Patterning in Self-Organized Human Ectoderm Tissues[J]. Matter,2020,2(6):1621-1638.

Xie，Tianfa,Kang，Jiming,Pak，Chang Hui,Yuan，Hongyan,&Sun，Yubing.(2020).Temporal Modulations of NODAL, BMP, and WNT Signals Guide the Spatial Patterning in Self-Organized Human Ectoderm Tissues.Matter,2(6),1621-1638.

Xie，Tianfa,et al."Temporal Modulations of NODAL, BMP, and WNT Signals Guide the Spatial Patterning in Self-Organized Human Ectoderm Tissues".Matter 2.6(2020):1621-1638.