Psoralen as an interstrand DNA crosslinker in the selection of DNA-Encoded dynamic chemical library

Zhou，Yu1; Peng，Jianzhao1,2; Shen，Wenyin1; Li，Xiaoyu1

2020

10.1016/j.bbrc.2020.04.033

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   影响因子和分区

0006-291X

1090-2104

DNA-encoded chemical library (DEL) has emerged as a powerful technology for ligand discovery in biomedical research. Recently, we have developed a DNA-encoded dynamic library (DEDL) approach by incorporating the concept of dynamic combinatorial library (DCL) with DELs. DEDL has shown excellent potential in ligand discovery towards a variety of protein targets. However, the requirement of having a pair of unnatural p-stilbazoles as the interstrand DNA crosslinker has limited the chemical diversity of DEDLs. Here, we replaced p-stilbazole with psoralen (PS) and tested the feasibility of psoralen as the crosslinker in DEDL selection. Since psoralen is commercially available and does not require any special crosslinking partner, existing DELs may be directly used to create high-diversity DEDLs. This study is expected to greatly facilitate the development of DEDLs as a versatile tool in drug discovery.

DNA-Encoded chemical library Drug discovery Dynamic combinatorial library High throughput screening Psoralen

SCI

英语

其他

Research Grants Council of the HKSAR[AoE/P-705/16][17321916][17302817][17301118][17111319] ; NSFC of China[21572014][21877093][91953119]

Biochemistry & Molecular Biology ; Biophysics

Biochemistry & Molecular Biology ; Biophysics

WOS:000583921700004

ACADEMIC PRESS INC ELSEVIER SCIENCE

BIOLOGY & BIOCHEMISTRY

2-s2.0-85083850126

Scopus

1.Department of Chemistry and State Key Laboratory of Synthetic Chemistry,The University of Hong Kong,Hong Kong SAR,Pokfulam Road,Hong Kong
2.Department of Chemistry,Southern University of Science and Technology,Shenzhen,1088 Xueyuan Road,518055,China

Zhou，Yu,Peng，Jianzhao,Shen，Wenyin,et al. Psoralen as an interstrand DNA crosslinker in the selection of DNA-Encoded dynamic chemical library[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2020,533(2):215-222.

Zhou，Yu,Peng，Jianzhao,Shen，Wenyin,&Li，Xiaoyu.(2020).Psoralen as an interstrand DNA crosslinker in the selection of DNA-Encoded dynamic chemical library.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,533(2),215-222.

Zhou，Yu,et al."Psoralen as an interstrand DNA crosslinker in the selection of DNA-Encoded dynamic chemical library".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 533.2(2020):215-222.