Target identification of natural medicine with chemical proteomics approach: probe synthesis, target fishing and protein identification

Chen, Xiao1,2,3; Wang, Yutong1,2; Ma, Nan4,5; Tian, Jing1,2; Shao, Yurou1,2; Zhu, Bo1,2,3; Wong, Yin Kwan4,5,6; Liang, Zhen6; Zou, Chang6; Wang, Jigang4,5,6,7

2020-05-21

10.1038/s41392-020-0186-y

Signal Transduction and Targeted Therapy   影响因子和分区

2095-9907

2059-3635

Natural products are an important source of new drugs for the treatment of various diseases. However, developing natural product-based new medicines through random moiety modification is a lengthy and costly process, due in part to the difficulties associated with comprehensively understanding the mechanism of action and the side effects. Identifying the protein targets of natural products is an effective strategy, but most medicines interact with multiple protein targets, which complicate this process. In recent years, an increasing number of researchers have begun to screen the target proteins of natural products with chemical proteomics approaches, which can provide a more comprehensive array of the protein targets of active small molecules in an unbiased manner. Typically, chemical proteomics experiments for target identification consist of two key steps: (1) chemical probe design and synthesis and (2) target fishing and identification. In recent decades, five different types of chemical proteomic probes and their respective target fishing methods have been developed to screen targets of molecules with different structures, and a variety of protein identification approaches have been invented. Presently, we will classify these chemical proteomics approaches, the application scopes and characteristics of the different types of chemical probes, the different protein identification methods, and the advantages and disadvantages of these strategies.

SCI

英语

通讯

National Natural Science Foundation of China[81903588][81803456][81841001] ; Major National Science and Technology Program of China for Innovative Drug[2017ZX09101002001-001-05] ; Fundamental Research Funds for the Central Public Welfare Research Institutes[ZXKT18003] ; Natural Science Foundation of Jiangsu Province (China)[BK20190799] ; Science and Technology Foundation of Shenzhen[JCYJ20180305164128430] ; International Cooperation Foundation of Shenzhen[GJHZ20180928171602104] ; Shenzhen Economic and Information Committee "Innovation Chain and Industry Chain" integration special support plan project[20180225112449943]

Biochemistry & Molecular Biology ; Cell Biology

Biochemistry & Molecular Biology ; Cell Biology

WOS:000537049300002

NATURE PUBLISHING GROUP

Web of Science

1.Nanjing Univ Chinese Med, Sch Med & Holist Integrat Med, Nanjing 210023, Peoples R China
2.Nanjing Univ Chinese Med, Coll Pharm, Nanjing 210023, Peoples R China
3.China Pharmaceut Univ, Sch Biopharm, Nanjing 210009, Peoples R China
4.China Acad Chinese Med Sci, Artemisinin Res Ctr, Beijing 100700, Peoples R China
5.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China
6.Jinan Univ, Southern Univ Sci & Technol, Clin Med Coll 2, Affiliated Hosp 1,Shenzhen Peoples Hosp, Shenzhen 518020, Peoples R China
7.Guangxi Med Univ, Sch Publ Hlth, Dept Toxicol, Nanning 530021, Peoples R China

Chen, Xiao,Wang, Yutong,Ma, Nan,et al. Target identification of natural medicine with chemical proteomics approach: probe synthesis, target fishing and protein identification[J]. Signal Transduction and Targeted Therapy,2020,5(1).

Chen, Xiao.,Wang, Yutong.,Ma, Nan.,Tian, Jing.,Shao, Yurou.,...&Wang, Jigang.(2020).Target identification of natural medicine with chemical proteomics approach: probe synthesis, target fishing and protein identification.Signal Transduction and Targeted Therapy,5(1).

Chen, Xiao,et al."Target identification of natural medicine with chemical proteomics approach: probe synthesis, target fishing and protein identification".Signal Transduction and Targeted Therapy 5.1(2020).