Alternative Splicing of OsRAD1 Defines C-Terminal Domain Essential for Protein Function in Meiosis

Yuan Shuting1,2,3; Xu Chunjue2; Yan Wei1,2; Chang Zhenyi1,2; Deng Xingwang2,3; Chen Zhufeng2; Wu Jianxin1; Tang Xiaoyan1,2

2020-07

10.1016/j.rsci.2020.05.005

Rice Science   影响因子和分区

1672-6308

1876-4762

Alternative splicing can generate multiple mRNAs that differ in their untranslated regions or coding sequences, and these differences might affect mRNA stability or result in different protein isoforms with diverse functions and/or localizations. In this study, we isolated a sterile mutant in rice with abnormal meiosis of microspore mother cells and megaspore mother cells that carried a point mutation in OsRAD1 gene. Cloning of OsRAD1 cDNAs revealed three transcript variants, named as OsRAD1.1, OsRAD1.2 and OsRAD1.3, respectively, which were derived from alternative splicing of the last intron. Proteins derived from the three transcripts were mostly identical except the difference in the very C-terminal domain. The three transcripts exhibited similar expression patterns in various tissues, but the expression level of OsRAD1.1 was the highest. Specific knockout of OsRAD1.1 led to sterility, while knockout of OsRAD1.2 and OsRAD1.3 together did not change the plant fertility. Overexpression of OsRAD1.2 and OsRAD1.3 cDNAs in OsRAD1.1-specific mutant did not complement the plant fertility. Yeast two-hybrid assay showed that OsRAD1.1, but not OsRAD1.2 and OsRAD1.3, interacted with the three other meiosis proteins OsHUS1, OsRAD9 and OsRAD17, suggesting that the C-terminal domain of OsRAD1.1 is critical for the protein function.

Oryza sativa OsRAD1 alternative splicing meiosis protein interaction fertility sterility

SCI

英语

其他

Natural Science Foundation of Guangdong Province[B030308008][2017A030310500][A03013104] ; National Key Research and Development Plan Program[2016YFD0101801][2016YFD0100406] ; Shenzhen Commission on Innovation and Technology Programs[JCYJ20160229204920363] ; Guangzhou Science and Technology Innovation Commission[201804010034] ; National Natural Science Foundation of China[31500254]

Agriculture ; Plant Sciences

Agronomy ; Plant Sciences

WOS:000541137500005

ELSEVIER

Web of Science

1.South China Normal Univ, Sch Life Sci, Guangdong Prov Key Lab Biotechnol Plant Dev, Guangzhou 510631, Peoples R China
2.Shenzhen Inst Mol Crop Design, Shenzhen 518107, Peoples R China
3.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen 518055, Peoples R China

Yuan Shuting,Xu Chunjue,Yan Wei,et al. Alternative Splicing of OsRAD1 Defines C-Terminal Domain Essential for Protein Function in Meiosis[J]. Rice Science,2020,27(4):289-301.

Yuan Shuting.,Xu Chunjue.,Yan Wei.,Chang Zhenyi.,Deng Xingwang.,...&Tang Xiaoyan.(2020).Alternative Splicing of OsRAD1 Defines C-Terminal Domain Essential for Protein Function in Meiosis.Rice Science,27(4),289-301.

Yuan Shuting,et al."Alternative Splicing of OsRAD1 Defines C-Terminal Domain Essential for Protein Function in Meiosis".Rice Science 27.4(2020):289-301.