Upconversion Nonlinear Structured Illumination Microscopy

Liu, Baolei1,3; Chen, Chaohao1; Di, Xiangjun1; Liao, Jiayan1; Wen, Shihui1; Su, Qian Peter1; Shan, Xuchen1; Xu, Zai-Quan3; Ju, Lining Arnold4,5,6; Mi, Chao1; Wang, Fan1; Jin, Dayong1,2

2020-07-08

10.1021/acs.nanolett.0c00448

NANO LETTERS   影响因子和分区

1530-6984

1530-6992

Video-rate super-resolution imaging through biological tissue can visualize and track biomolecule interplays and transportations inside cellular organisms. Structured illumination microscopy allows for wide-field super resolution observation of biological samples but is limited by the strong extinction of light by biological tissues, which restricts the imaging depth and degrades its imaging resolution. Here we report a photon upconversion scheme using lanthanide-doped nanoparticles for wide-field super-resolution imaging through the biological transparent window, featured by near-infrared and low-irradiance nonlinear structured illumination. We demonstrate that the 976 nm excitation and 800 nm upconverted emission can mitigate the aberration. We found that the nonlinear response of upconversion emissions from single nanoparticles can effectively generate the required high spatial frequency components in the Fourier domain. These strategies lead to a new modality in microscopy with a resolution below 131 nm, 1/7th of the excitation wavelength, and an imaging rate of 1 Hz.

SIM super-resolution nonlinear NIR upconversion nanoparticles deep tissue

SCI ; EI

英语

NI论文

通讯

Australian Research Council Discovery Projects[DP190101058][DP200101970] ; Shenzhen S c i e n c e and Technology Program[KQTD20170810110913065] ; Australia China Science and Research Fund Joint Research Centre for POCT[ACSRF65827] ; Australian Research Council DECRA fellowships[DE200100074][DE190100609] ; China Scholarship Council[201706020170][201607950009][201706170028][201508530231][201708200004]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000548893200011

AMER CHEMICAL SOC

20203108983243

Nanoparticles ; Biomolecules ; Infrared devices ; Optical resolving power

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Light/Optics:741.1 ; Nanotechnology:761 ; Biochemistry:801.2 ; Solid State Physics:933

MATERIALS SCIENCE

Web of Science

1.Univ Technol Sydney, Inst Biomed Mat & Devices IBMD, Fac Sci, Sydney, NSW 2007, Australia
2.Southern Univ Sci & Technol, Dept Biomed Engn, UTS SUStech Joint Res Ctr Biomed Mat & Devices, Shenzhen 518055, Peoples R China
3.Univ Technol Sydney, Fac Sci, Sch Math & Phys Sci, Sydney, NSW 2007, Australia
4.Univ Sydney, Sch Biomed Engn, Fac Engn, Camperdown, NSW 2006, Australia
5.Univ Sydney, Charles Perkins Ctr, Camperdown, NSW 2006, Australia
6.Heart Res Inst, Newtown, NSW 2042, Australia

Liu, Baolei,Chen, Chaohao,Di, Xiangjun,et al. Upconversion Nonlinear Structured Illumination Microscopy[J]. NANO LETTERS,2020,20(7):4775-4781.

Liu, Baolei.,Chen, Chaohao.,Di, Xiangjun.,Liao, Jiayan.,Wen, Shihui.,...&Jin, Dayong.(2020).Upconversion Nonlinear Structured Illumination Microscopy.NANO LETTERS,20(7),4775-4781.

Liu, Baolei,et al."Upconversion Nonlinear Structured Illumination Microscopy".NANO LETTERS 20.7(2020):4775-4781.