Emerging technologies for profiling extracellular vesicle heterogeneity

Huang, Guan1; Lin, Gungun1,2; Zhu, Ying3; Duan, Wei4; Jin, Dayong1,2,5

2020-07-21

10.1039/d0lc00431f

LAB ON A CHIP   影响因子和分区

1473-0197

1473-0189

Extracellular vesicles (EVs) are membrane-bound vesicles secreted by most cell types and exist in virtually all bodily fluids. They carry on a wealth of proteomic and genetic information including proteins, lipids, miRNAs, mRNA, non-coding RNA and other molecules from parental cells. Increasing evidence shows that within populations of EVs, their biogenesis, physical characteristics (e.g. size, density, morphology) and cargos (e.g. protein, lipid content, nucleic acids) may vary substantially, which accordingly change their biological properties. To fully exploit the potential of EVs, it requires qualified methods to profile EV heterogeneity. In this review, we survey recent approaches for EV isolation with innovative discoveries in heterogeneity. The main challenges in EV heterogeneity research are identified, and the roles of single cell EV profiling and single EV imaging are highlighted. We further discuss promising opportunities for resolving the underlying complexity of EV heterogeneity.

SCI ; EI

英语

其他

ARC Industry Transformational Research Hub Scheme[IH150100028] ; NHMRC Fellowship[GNT1160635] ; Oversea Study Program of Guangzhou Elite Project[JY201840] ; Cancer Institute NSW Early Career Fellowship[2017/EFC012] ; Ovarian Cancer Research Foundation[GA-2019-13]

Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation

Biochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation

WOS:000548638600013

ROYAL SOC CHEMISTRY

20203008977469

Genes ; RNA

Biological Materials and Tissue Engineering:461.2 ; Organic Compounds:804.1

CHEMISTRY

Web of Science

1.Univ Technol Sydney, Fac Sci, Inst Biomed Mat & Devices, Ultimo, NSW 2007, Australia
2.Univ Technol Sydney, Fac Sci, ARC Res Hub Integrated Device End User Anal Low L, Sydney, NSW 2007, Australia
3.UNSW Sydney, St George & Sutherland Clin Sch, Sydney, NSW 2052, Australia
4.Deakin Univ, Sch Med, Waurn Ponds, Vic 3216, Australia
5.Southern Univ Sci & Technol, Dept Biomed Engn, UTS SUStech Joint Res Ctr Biomed Mat & Devices, Shenzhen, Peoples R China

Huang, Guan,Lin, Gungun,Zhu, Ying,et al. Emerging technologies for profiling extracellular vesicle heterogeneity[J]. LAB ON A CHIP,2020,20(14):2423-2437.

Huang, Guan,Lin, Gungun,Zhu, Ying,Duan, Wei,&Jin, Dayong.(2020).Emerging technologies for profiling extracellular vesicle heterogeneity.LAB ON A CHIP,20(14),2423-2437.

Huang, Guan,et al."Emerging technologies for profiling extracellular vesicle heterogeneity".LAB ON A CHIP 20.14(2020):2423-2437.