RSU-1 Maintains Integrity of Caenorhabditis elegans Vulval Muscles by Regulating alpha-Actinin

Wang, Xinyan1,3; Huang, Shuai1; Zheng, Cunni1; Ge, Wei3; Wu, Chuanyue4; Tse, Yu Chung1,2

2020-07

10.1534/g3.120.401185

G3-Genes Genomes Genetics   影响因子和分区

2160-1836

Egg-laying behavior in Caenorhabditis elegans is a well-known model for investigating fundamental cellular processes. In egg-laying, muscle contraction is the relaxation of the vulval muscle to extrude eggs from the vulva. Unlike skeletal muscle, vulval muscle lacks visible striations of the sarcomere. Therefore, vulval muscle must counteract the mechanical stress, caused by egg extrusion and body movement, from inducing cell-shape distortion by maintaining its cytoskeletal integrity. However, the underlying mechanisms that regulate the cellular integrity in vulval muscles remain unclear. Here, we demonstrate that C. elegans egg-laying requires proper vulval muscle 1 (vm1), in which the actin bundle organization of vm1 muscles is regulated by Ras suppressor protein 1 (RSU-1). In the loss of RSU-1, as well as Ras(LET-60) overactivation, blister-like membrane protrusions and disorganized actin bundles were observed in the vm1 muscles. Moreover, Ras(LET-60) depletion diminished the defected actin-bundles in rsu-1 mutant. These results reveal the genetic interaction of RSU-1 and Ras(LET-60) in vivo. In addition, our results further demonstrated that the fifth to seventh leucine-rich region of RSU-1 is required to promote actin-bundling protein, alpha-actinin, for actin bundle stabilization in the vm1 muscles. This expands our understanding of the molecular mechanisms of actin bundle organization in a specialized smooth muscle.

alpha-actinin Egg-laying Ras RSU-1 Vulval muscle cells

SCI

英语

第一 ; 通讯

Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research[2017B030301018] ; Shenzhen Key Laboratory of Cell Microenvironment[ZDSYS20140509142721429] ; National Natural Science Foundation of China[31471311][31671409] ; Shenzhen Science and Technology Innovation Commission[JCYJ20170307105005654]

Genetics & Heredity

Genetics & Heredity

WOS:000551887900034

GENETICS SOCIETY AMERICA

Web of Science

1.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Guangdong Prov Key Lab Cell Microenvironm & Dis R, Shenzhen Key Lab Cell Microenvironm, Shenzhen 518055, Peoples R China
3.Univ Macau, Fac Hlth Sci, Ctr Reprod Dev & Aging, Macau, Peoples R China
4.Univ Pittsburgh, Sch Med, Dept Pathol, Pittsburgh, PA 15261 USA

Wang, Xinyan,Huang, Shuai,Zheng, Cunni,et al. RSU-1 Maintains Integrity of Caenorhabditis elegans Vulval Muscles by Regulating alpha-Actinin[J]. G3-Genes Genomes Genetics,2020,10(7):2507-2517.

Wang, Xinyan,Huang, Shuai,Zheng, Cunni,Ge, Wei,Wu, Chuanyue,&Tse, Yu Chung.(2020).RSU-1 Maintains Integrity of Caenorhabditis elegans Vulval Muscles by Regulating alpha-Actinin.G3-Genes Genomes Genetics,10(7),2507-2517.

Wang, Xinyan,et al."RSU-1 Maintains Integrity of Caenorhabditis elegans Vulval Muscles by Regulating alpha-Actinin".G3-Genes Genomes Genetics 10.7(2020):2507-2517.