Development of a molecular K(+)probe for colorimetric/fluorescent/photoacoustic detection of K+

Ning, Juewei1; Lin, Xiangwei2,3; Su, Fengyu1,4; Sun, Aihui5; Liu, Hongtian1; Luo, Jingdong6; Wang, Lidai2,3; Tian, Yanqing1

2020-07-25

10.1007/s00216-020-02826-y

ANALYTICAL AND BIOANALYTICAL CHEMISTRY   影响因子和分区

1618-2642

1618-2650

The potassium ion (K+) plays significant roles in many biological processes. To date, great efforts have been devoted to the development of K+ sensors for colorimetric, fluorescent, and photoacoustic detection of K+ separately. However, the development of molecular K+ probes for colorimetric detection of urinary K+, monitoring K+ fluxes in living cells by fluorescence imaging, and photoacoustic imaging of K+ dynamics in deep tissues still remains an open challenge. Herein, we report the first molecular K+ probe ( NK2) for colorimetric, fluorescent, and photoacoustic detection of K+. NK2 is composed of 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) as the chromophore and phenylazacrown-6-lariat ether (ACLE) as the K+ recognition unit. Predominate features of NK2 include a short synthetic procedure, high K+ selectivity, large detection range (5-200 mM), and triple-channel detection manner. NK2 shows good response to K+ with obvious color changes, fluorescence enhancements (about threefold), and photoacoustic intensity changes. The existence of other metal ions (including Na+, Mg2+, Ca2+, Fe2+) and pH changes (6.5-9.0) have no obvious influence on K+ sensing of NK2. Portable test strips stained by NK2 can be used to qualitatively detect urinary K+ by color changes for self-diagnosis of diseases induced by high levels of K+. NK2 can be utilized to monitor K+ fluxes in living cells by fluorescent imaging. We also find its excellent performance in photoacoustic imaging of different K+ concentrations in the mouse ear. NK2 is the first molecular K+ probe for colorimetric, fluorescent, and photoacoustic detection of K+ in urine, in living cells, and in the mouse ear. The development of NK2 will broaden K+ probes' design and extend their applications to different fields.

Potassium ion probe Colorimetric detection Fluorescent imaging Photoacoustic imaging K+ fluxes

SCI ; EI

英语

第一 ; 通讯

National Natural Science Foundation of China[21774054][21574061][81627805][61805102] ; Shenzhen Fundamental Research Programs[JCYJ20170412152922553] ; Research Grants Council of the Hong Kong Special Administrative Region[21205016][11215817][11101618] ; Science Technology and Innovation Commission of Shenzhen Municipality[JCYJ20160329150236426][JCYJ20170413140519030]

Biochemistry & Molecular Biology ; Chemistry

Biochemical Research Methods ; Chemistry, Analytical

WOS:000552266600001

SPRINGER HEIDELBERG

20203108991762

Metal ions ; Colorimetry ; Diagnosis ; Chromophores ; Color ; Potassium ; Fluorescence imaging

Medicine and Pharmacology:461.6 ; Metallurgy:531.1 ; Alkali Metals:549.1 ; Light/Optics:741.1 ; Imaging Techniques:746 ; Organic Compounds:804.1 ; Optical Variables Measurements:941.4

CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China
2.City Univ Hong Kong, Dept Biomed Engn, Kowloon, 83 Tat Chee Ave, Hong Kong 999077, Peoples R China
3.City Univ Hong Kong, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
4.Southern Univ Sci & Technol, Acad Adv Interdisciplinary Studies, Shenzhen 518055, Peoples R China
5.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China
6.City Univ Hong Kong, Dept Chem, Kowloon, 83 Tat Chee Ave, Hong Kong 999077, Peoples R China

Ning, Juewei,Lin, Xiangwei,Su, Fengyu,et al. Development of a molecular K(+)probe for colorimetric/fluorescent/photoacoustic detection of K+[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2020,412(25):6947-6957.

Ning, Juewei.,Lin, Xiangwei.,Su, Fengyu.,Sun, Aihui.,Liu, Hongtian.,...&Tian, Yanqing.(2020).Development of a molecular K(+)probe for colorimetric/fluorescent/photoacoustic detection of K+.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,412(25),6947-6957.

Ning, Juewei,et al."Development of a molecular K(+)probe for colorimetric/fluorescent/photoacoustic detection of K+".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 412.25(2020):6947-6957.