| 题名 | Accurate 4Pi single-molecule localization using an experimental PSF model |
| 作者 | |
| 通讯作者 | Ries, Jonas |
| 发表日期 | 2020-07
|
| DOI | |
| 发表期刊 | |
| ISSN | 0146-9592
|
| EISSN | 1539-4794
|
| 卷号 | 45期号:13页码:3765-3768 |
| 摘要 | Interferometric single-molecule localization microscopy (iPALM, 4Pi-SMS) uses multiphase interferometry to localize single fluorophores and achieves nanometer isotropic resolution in 3D. The current data analysis workflow, however, fails to reach the theoretical resolution limit due to the suboptimal localization algorithm. Here, we develop a method to fit an experimentally derived point spread. function (PSF) model to the interference 4Pi-PSF. As the interference phase is not fixed with respect to the shape of the PSF, we decoupled the phase term in the model from the 3D position of the PSF. The fitter can reliably infer the interference period even without introducing astigmatism, reducing the complexity of the microscope. Using a spline-interpolated experimental PSF model and by fitting all phase images globally, we show on simulated data that we can achieve the theoretical limit of 3D resolution, the Cramer-Rao lower bound (CRLB), also for the 4Pi microscope. (C) 2020 Optical Society of America |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
|
| 学校署名 | 第一
|
| 资助项目 | EMBL Interdisciplinary Postdoc Programme (EIPOD) under Marie Curie Actions COFUND; Common Fund[U01 EB021223]
; European Research Council[ERCCoG-724489]
|
| WOS研究方向 | Optics
|
| WOS类目 | Optics
|
| WOS记录号 | WOS:000546808900107
|
| 出版者 | |
| EI入藏号 | 20202908949352
|
| EI主题词 | Molecules
; 3D modeling
; Interferometry
|
| EI分类号 | Data Processing and Image Processing:723.2
; Light/Optics:741.1
; Atomic and Molecular Physics:931.3
; Optical Variables Measurements:941.4
|
| ESI学科分类 | PHYSICS
|
| 来源库 | Web of Science
|
| 引用统计 |
被引频次[WOS]：11
|
| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/141466 |
| 专题 | 工学院_生物医学工程系 |
| 作者单位 | 1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China 2.EMBL, Cell Biol & Biophys, D-69117 Heidelberg, Germany 3.Yale Sch Med, Dept Cell Biol, New Haven, CT 06511 USA 4.Yale Univ, Dept Biomed Engn, New Haven, CT 06511 USA |
| 第一作者单位 | 生物医学工程系 |
| 第一作者的第一单位 | 生物医学工程系 |
| 推荐引用方式 GB/T 7714 |
Li, Yiming,Buglakova, Elena,Zhang, Yongdeng,et al. Accurate 4Pi single-molecule localization using an experimental PSF model[J]. OPTICS LETTERS,2020,45(13):3765-3768.
|
| APA |
Li, Yiming,Buglakova, Elena,Zhang, Yongdeng,Thevathasan, Jervis Vermal,Bewersdorf, Joerg,&Ries, Jonas.(2020).Accurate 4Pi single-molecule localization using an experimental PSF model.OPTICS LETTERS,45(13),3765-3768.
|
| MLA |
Li, Yiming,et al."Accurate 4Pi single-molecule localization using an experimental PSF model".OPTICS LETTERS 45.13(2020):3765-3768.
|
| 条目包含的文件 | 条目无相关文件。 | |||||
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