Surface-modified mesoporous nanofibers for microfluidic immunosensor with an ultra-sensitivity and high signal-to-noise ratio

Li，Zulan1; Liu，Ye2; Chen，Xingming3; Cao，Hongyan1; Shen，Haiying4; Mou，Lei4; Deng，Xinli1; Jiang，Xingyu4; Cong，Yulong1

2020-10-15

10.1016/j.bios.2020.112444

BIOSENSORS & BIOELECTRONICS   影响因子和分区

0956-5663

1873-4235

How to balance the sensitivity and signal-to-noise ratio of immunosensor remains many challenges during various diseases diagnosis. Here we develop a new microfluidic immunosensor based on surface-modified mesoporous nanofibers, and simultaneously realize an ultra-sensitivity and high signal-to-noise ratio for the detection of multiple biomarkers. In the current study, we fabricated titanium dioxide (TiO)-based mesoporous electrospinning nanofibers, and modified nanofiber surface with both octadecylphosphonic acid (OPA) and poly(ethylene oxide)-poly(propylene oxide) triblock copolymer (PEO-PPO-PEO). Such nanofibers as solid substrate are covered on microfluidic channels. The porosity of our nanofibers dramatically increased the adsorption capability of antibodies, realizing an ultra sensitivity of biomarker detection. PEO-PPO-PEO modification can significantly block non-specific absorptions, obtaining a satisfied signal-to-noise ratio. For the detection of HIV p24 and interleukin 5 (IL-5), our immunosensor increased 6.41 and 6.93 fold in sensitivity and improved 504.66% and 512.80% in signal-to-noise ratio, in compared with gold standard immunoassay (ELISA) used in the clinic. Our immunosensor also broaden the linear range for the detection of HIV p24 (0.86–800 pg/ml) and IL-5 (0.70–800 pg/ml), in compared with ELISA which is 5.54–500 pg/ml for HIV p24 and 4.84–500 pg/ml for IL-5. Our work provided a guideline for the construction of advanced point-of-care immunosensor with an ultra-sensitivity and high signal-to-noise ratio for disease diagnosis.

Mesoporous nanofibers Microfluidic immunosensor Sensitivity Signal-to-noise ratio Surface modification

SCI ; EI

英语

其他

National Natural Science Foundation of China[21874033][21535001][81730051][21761142006] ; Leading Medical Talents Program of Health Commission of Yunnan Province[L2018013] ; Chinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Science[2020-I2M-2-014] ; National Key R&D Program of China[2018YFA0902600][2017YFA0205901] ; Chinese Academy of Sciences[QYZDJ-SSW-SLH039][121D11KYSB20170026][XDA16020902]

Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics

Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology

WOS:000566449100001

ELSEVIER ADVANCED TECHNOLOGY

20203209008144

Biomarkers ; Diagnosis ; Microfluidics ; Nanofibers ; Chemical detection ; Polyethylene oxides ; Mesoporous materials ; Diseases ; Ethylene ; Titanium dioxide

Medicine and Pharmacology:461.6 ; Microfluidics:632.5.1 ; Information Theory and Signal Processing:716.1 ; Nanotechnology:761 ; Chemistry:801 ; Organic Compounds:804.1 ; Inorganic Compounds:804.2 ; Organic Polymers:815.1.1 ; Solid State Physics:933

CHEMISTRY

2-s2.0-85088863542

Scopus

1.PLA Medical College and Clinical Laboratory of Second Medical Center of PLA General Hospital,Beijing,No. 28 Fuxing Road,100853,China
2.Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Kunming,650000,China
3.Laboratory Department of PLA Strategic Support Force Characteristic Medical Center,Beijing,100101,China
4.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,No. 1088 Xueyuan Rd, Nanshan District,518055,China

Li，Zulan,Liu，Ye,Chen，Xingming,et al. Surface-modified mesoporous nanofibers for microfluidic immunosensor with an ultra-sensitivity and high signal-to-noise ratio[J]. BIOSENSORS & BIOELECTRONICS,2020,166.

Li，Zulan.,Liu，Ye.,Chen，Xingming.,Cao，Hongyan.,Shen，Haiying.,...&Cong，Yulong.(2020).Surface-modified mesoporous nanofibers for microfluidic immunosensor with an ultra-sensitivity and high signal-to-noise ratio.BIOSENSORS & BIOELECTRONICS,166.

Li，Zulan,et al."Surface-modified mesoporous nanofibers for microfluidic immunosensor with an ultra-sensitivity and high signal-to-noise ratio".BIOSENSORS & BIOELECTRONICS 166(2020).