基于拉曼光谱的微生物代谢特征及抗生素抗性研究

STUDY ON METABOLIC CHARACTERISTICS AND ANTIBIOTIC RESISTANCE OF BACTERIA BASED ON THE RAMAN SPECTRUM

王艺

11649003

博士

环境科学与工程

郑春苗

2020-06-05

2020-08-02

哈尔滨工业大学

深圳

微生物是生态环境系统的重要组成部分，是环境科学领域重要的研究内容。目前对于环境微生物的研究方法主要为传统培养学方法和基因组学方法。然而，在现有技术条件下，微生物可分离培养率一般低于1%；而且微生物分离培养后会改变其原始生活环境，面临难以分析微生物原始代谢特性的瓶颈问题。基因组学方法无法确定微生物个体的生理和代谢特征，因此难以建立微生物特定表现型与基因型相关关系。因此，在保证微生物原始生活环境的前提下，非破坏性地对微生物的代谢特征、表现型及基因型进行检测，对环境微生物领域的研究具有重要意义。

Microorganisms are an important part of the ecosystem and an important research content in the field of environmental science. At present, the research methods for environmental microorganisms are mainly traditional culture methods and genomics methods. However, under the existing technical conditions, the survival rate of microorganisms is generally less than 1% after separation. The separation of microorganisms usually changes their original living environment, facing with a bottleneck that is difficult to analyze the original metabolic properties of microorganisms. Genomics methods cannot determine the physiological and metabolic characteristics of microorganisms, so it is difficult to establish the correlation between specific phenotypes and genotypes of microorganisms. Therefore, on the premise of ensuring the original living environment of microorganisms, non-destructive in-situ detection of the metabolic characteristics, phenotypes and genotypes of microorganisms is of great significance to the research in the field of environmental microorganisms.

拉曼光谱 单细胞分选 代谢特征 微生物表现型与基因型 抗生素耐药性

Raman spectrum single cell ejection metabolism genotype and phenotype antibiotic resistance

中文

联合培养

南方科技大学

王艺. 基于拉曼光谱的微生物代谢特征及抗生素抗性研究[D]. 深圳. 哈尔滨工业大学,2020.