Dvl家族在斑马鱼侧线毛细胞再生调控中的作用

THE ROLES OF DISHEVELED FAMILY IN THE REGULATION OF HAIR CELL REGENERATION OF ZEBRAFISH LATERAL LINE

王鹏

11849364

硕士

生物学

刘东

2020-06-03

2020-02-02

哈尔滨工业大学

深圳

成体哺乳动物的内耳无法再生毛细胞。人的毛细胞一旦受损或死亡便会造成永久性的听觉和平衡觉的功能异常，甚至会导致个体失聪、失衡。但是自然界中鸟类、两栖类和硬骨鱼类等非哺乳类脊椎动物的内耳及侧线等感觉系统却能终生再生毛细胞。因此，研究这些非哺乳动物的毛细胞再生机制，将有助于我们了解哺乳动物无法修复内耳毛细胞损伤的内在原因，进而帮助寻找诱导毛细胞再生的治疗手段。本课题以斑马鱼侧线神经丘为研究对象，从一个侧线毛细胞发育正常但再生异常的突变体sim1begfpEt 入手，利用胚胎活体成像、实时荧光定量PCR、显微注射、基因编辑、免疫共沉淀等技术探讨该突变体在顺铂损伤后，侧线再生毛细胞异常的遗传机制。

The inner ear of adult mammals can’t regenerate the hair cells (HCs) once they are damaged or dead, causing permanent hearing and balance dysfunction, or even the deafness and a loss of balance. In contrast, non-mammalian vertebrates like birds, amphibians and teleosts can regenerate their sensory HCs throughout their lives, in the inner ear or lateral line system. To understand why mammals fail to regenerate the HCs in damaged inner ear, studying the mechanisms underlying HC regeneration in the non-mammalian animals would be helpful for us to induce proper HCs regeneration in humans. In this project, we have chosen posterior lateral line (pLL) neuromasts of a zebrafish sim1begfpEt mutant and techniques such as live imaging, quantitative PCR, genome editing, and co-immunoprecipitation to investigate why the mutant develops normal pLL neuromast HCs but fails to regenerate nascent HCs once the pLL neuromasts are damaged by cisplatin.

毛细胞损伤 Wnt信号通路 sim1b Dvl家族 毛细胞再生

hair cell damage Wnt signaling pathway sim1b Dvl family hair cell regeneration

中文

联合培养

南方科技大学

王鹏. Dvl家族在斑马鱼侧线毛细胞再生调控中的作用[D]. 深圳. 哈尔滨工业大学,2020.