G protein-coupled estrogen receptor 1 mediates estrogen effect in red common carp (Cyprinus carpio)

Liu，Shuai1; Chen，Fangyi2; Zhang，Yuanyuan1; Cai，Ling3; Qiu，Wenhui4; Yang，Ming1,5

2021

10.1016/j.cbpc.2020.108868

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY   影响因子和分区

1532-0456

1878-1659

G protein-coupled estrogen receptor 1 (GPER1) plays a crucial role in the regulation of non-genomic estrogen effect. However, the research about fish GPER1 is still limited. The present study aims to obtain the full-length sequence of gper1 from red common carp (Cyprinus carpio) and characterize its expression pattern, and to further explore its potential role in regulating the environmental estrogen induced immunotoxicity. We first cloned the full-length mRNA and genomic sequences of gper1 in C. carpio by PCR, and obtained a 1908 bp sequence with a 1062 bp open reading frame encoding GPER1 protein with 353 amino acids. Additionally, qRT-PCR showed that gper1 was expressed across different tissues in C. carpio, with the highest expression in the brain, which is similar to that in zebrafish. Moreover, applying a luciferase reporter system, we found that the promotor sequence of gper1 has strong activity, and similar to GPER1 in other animals, carp GPER1 also has seven-transmembrane domains, indicating its potential functions. We confirmed the binding ability of GPER1 with G1 and G15 in primary macrophages of C. carpio by testing the related gene expression levels after 6 h exposure, and similar to G1, bisphenol A (BPA), a typical environmental estrogen, could interact with GPER1 to increase the Ca concentration in macrophages treated for 30 min. Furthermore, inhibition of GPER1 with GPER1 antagonist G36 rescued the cellular immunotoxicity caused by BPA, which further suggested that carp GPER1 could mediate the estrogen effect. Our findings contribute to better understanding of the role of carp GPER1.

Estrogen effect GPER1 mRNA expression Red common carp

SCI

英语

其他

National Natural Science Foundation of China[31971523]

Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology

Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology

WOS:000588051700008

ELSEVIER SCIENCE INC

BIOLOGY & BIOCHEMISTRY

2-s2.0-85089686282

Scopus

1.School of Environmental and Chemical Engineering,Shanghai University,Shanghai,200444,China
2.State Key Laboratory of Marine Environmental Science,Xiamen University,Xiamen,361005,China
3.Third Institute of Oceanography,Ministry of Natural Resources,PRC,Xiamen,361005,China
4.State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control,School of Environmental Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
5.Shanghai Applied Radiation Institute,Shanghai University,Shanghai,200444,China

Liu，Shuai,Chen，Fangyi,Zhang，Yuanyuan,et al. G protein-coupled estrogen receptor 1 mediates estrogen effect in red common carp (Cyprinus carpio)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2021,239.

Liu，Shuai,Chen，Fangyi,Zhang，Yuanyuan,Cai，Ling,Qiu，Wenhui,&Yang，Ming.(2021).G protein-coupled estrogen receptor 1 mediates estrogen effect in red common carp (Cyprinus carpio).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,239.

Liu，Shuai,et al."G protein-coupled estrogen receptor 1 mediates estrogen effect in red common carp (Cyprinus carpio)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 239(2021).