The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity

Tran，Tuan Minh1; Ma，Zhiming1; Triebl，Alexander2; Nath，Sangeeta3,4; Cheng，Yingying5; Gong，Ben Qiang6; Han，Xiao1; Wang，Junqi7; Li，Jian Feng6; Wenk，Markus R.2; Torta，Federico2; Mayor，Satyajit3,8; Yang，Liang5,9; Miao，Yansong1,10

2020-10-01

10.26508/lsa.202000720

Life Science Alliance   影响因子和分区

2575-1077

2575-1077

Quorum sensing (QS) is a recognized phenomenon that is crucial for regulating population-related behaviors in bacteria. However, the direct specific effect of QS molecules on host biology is largely understudied. In this work, we show that the QS molecule DSF (cis-11-methyl-dodecenoic acid) produced by Xanthomonas campestris pv. campestris can suppress pathogen-associated molecular pattern-triggered immunity (PTI) in Arabidopsis thaliana, mediated by flagellin-induced activation of flagellin receptor FLS2. The DSF-mediated attenuation of innate immunity results from the alteration of FLS2 nanoclusters and endocytic internalization of plasma membrane FLS2. DSF altered the lipid profile of Arabidopsis, with a particular increase in the phytosterol species, which impairs the general endocytosis pathway mediated by clathrin and FLS2 nano-clustering on the plasma membrane. The DSF effect on receptor dynamics and host immune responses could be entirely reversed by sterol removal. Together, our results highlighted the importance of sterol homeostasis to plasma membrane organization and demonstrate a novel mechanism by which pathogenic bacteria use their communicating molecule to manipulate pathogen-associated molecular pattern-triggered host immunity.

SCI

英语

其他

Department of Biotechnology-Wellcome Trust Alliance[IA/M/15/1/502018] ; Nanyang Technological University NIMBELS grant[NIM/01/2016] ; Nanyang Technological University[M4081533]

Life Sciences & Biomedicine - Other Topics

Biology

WOS:000586255600007

LIFE SCIENCE ALLIANCE LLC

2-s2.0-85089610541

Scopus

1.School of Biological Sciences,Nanyang Technological University,Singapore
2.Department of Biochemistry,Singapore Lipidomics Incubator (SLING),Yoo Loo Lin School of Medicine,National University of Singapore,Singapore
3.Institute for Stem Cell Biology and Regenerative Medicine,Bangalore,India
4.Manipal Institute of Regenerative Medicine,Manipal Academy of Higher Education,Bangalore,India
5.Singapore Centre for Environmental Life Sciences Engineering,Nanyang Technological University,Singapore
6.School of Life Sciences,Sun Yat-sen University,Guangzhou,China
7.Department of Biology,Southern University of Science and Technology,Shenzhen,China
8.National Centre for Biological Sciences,Tata Institute for Fundamental Research,Bangalore,India
9.School of Medicine,Southern University of Science and Technology,Shenzhen,China
10.School of Chemical and Biomedical Engineering,Nanyang Technological University,Singapore

Tran，Tuan Minh,Ma，Zhiming,Triebl，Alexander,et al. The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity[J]. Life Science Alliance,2020,3(10).

Tran，Tuan Minh.,Ma，Zhiming.,Triebl，Alexander.,Nath，Sangeeta.,Cheng，Yingying.,...&Miao，Yansong.(2020).The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity.Life Science Alliance,3(10).

Tran，Tuan Minh,et al."The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity".Life Science Alliance 3.10(2020).