Fluorescent Bioconjugates for Super-Resolution Optical Nanoscopy

Liu，Zhihe1; Liu，Jie1,2; Wang，Xiaodong1; Mi，Feixue1; Wang，Dan3; Wu，Changfeng1

2020-08-19

10.1021/acs.bioconjchem.0c00320

BIOCONJUGATE CHEMISTRY   影响因子和分区

1043-1802

1520-4812

Fluorescent microscopy techniques are widely used in biological studies. However, the spatial resolution of fluorescent microscopy is restricted by the optical diffraction limit. In the past two decades, super-resolution imaging techniques with different principles have been invented to visualize biomolecules at nanometer scales. The development of nearly all these techniques is closely related to the advances in fluorescent probes. In particular, the intrinsic properties of fluorescent probes constrain the optimal imaging performance of super-resolution nanoscopy techniques. In this review, we summarized the recent progress in fluorescent probe bioconjugates for super-resolution imaging techniques. Examples of these bioconjugates include the widely used fluorescent proteins (FPs), organic dyes, quantum dots (Qdots), carbon dots (Cdots), upconversion nanoparticles (UCNPs), aggregation induced emission (AIE) nanoparticles, and polymer dots (Pdots). Based on the characteristics of the existing probes and their adaptability in current imaging methods, we provide a perspective for further development of fluorescent probes for super-resolution imaging.

SCI ; EI

英语

第一

Shenzhen Science and Technology Innovation Commission[KQTD20170810111314625] ; National Natural Science Foundation of China[81771930]

Biochemistry & Molecular Biology ; Chemistry

Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic

WOS:000563069900001

AMER CHEMICAL SOC

20220411528204

Biomolecules ; Diffraction ; Fluorescence ; Optical resolving power ; Probes ; Semiconductor quantum dots

Biology:461.9 ; Semiconductor Devices and Integrated Circuits:714.2 ; Light/Optics:741.1 ; Optical Devices and Systems:741.3 ; Biochemistry:801.2 ; Optical Instruments:941.3 ; Optical Variables Measurements:941.4

CHEMISTRY

2-s2.0-85089712976

Scopus

1.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,510855,China
2.Department of Biology,Hong Kong Baptist University,Hong Kong,999077,China
3.Key Laboratory of Functional Inorganic Material Chemistry,Ministry of Education,School of Chemistry and Materials Science,Heilongjiang University,Harbin,150080,China

Liu，Zhihe,Liu，Jie,Wang，Xiaodong,et al. Fluorescent Bioconjugates for Super-Resolution Optical Nanoscopy[J]. BIOCONJUGATE CHEMISTRY,2020,31(8):1857-1872.

Liu，Zhihe,Liu，Jie,Wang，Xiaodong,Mi，Feixue,Wang，Dan,&Wu，Changfeng.(2020).Fluorescent Bioconjugates for Super-Resolution Optical Nanoscopy.BIOCONJUGATE CHEMISTRY,31(8),1857-1872.

Liu，Zhihe,et al."Fluorescent Bioconjugates for Super-Resolution Optical Nanoscopy".BIOCONJUGATE CHEMISTRY 31.8(2020):1857-1872.