Dissemination of a ‘rare’ extended-spectrum β-lactamase gene blaSFO-1 mediated by epidemic clones of carbapenemase-producing Enterobacter hormaechei in China

Zhou，Kai1,2; Zhou，Yanzi2; Zhang，Chuqiu1; Song，Jingjie1; Cao，Xiaoli3; Yu，Xiao2; Shen，Ping2; Xiao，Yonghong2

2020-09-01

10.1016/j.ijantimicag.2020.106079

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS   影响因子和分区

0924-8579

1872-7913

An increasing trend of the coexistence of a rare extended-spectrum β-lactamase gene bla and carbapenemase genes in Enterobacteriaceae has recently been noted. This study aimed to determine the epidemiological and genetic characterisation of SFO-1-positive carbapenem-resistant Enterobacter cloacae complex (CREC) isolates. A total of 61 CREC clinical isolates were collected in the framework of a national surveillance for carbapenem-resistant Enterobacteriaceae during 2011–2015 in China. Seven SFO-1-positive CREC isolates (11.5%) were identified in four provinces, suggesting a wide dissemination of the bla gene among the CREC population in China. Five SFO-1-positive CREC isolates were further identified by screening 1625 genomes of E. cloacae complex strains retrieved from GenBank. The 12 SFO-1-positive CREC isolates were further identified as Enterobacter hormaechei, of which 10 belonged to epidemic clones (ST93, ST114 and ST418), indicating that these clones might largely contribute to the dissemination of bla. Phylogenomics analysis further identified the occurrence of clonal dissemination in the community setting. The bla-bearing plasmids were assigned to various incompatibility groups with highly diverse sizes (~104–370 kb), suggesting a wide vector range of bla. Two types of genetic context, with and without insertion sequence IS26, were identified for the bla gene. The genetic context flanked by IS26 was more prevalent, thus largely facilitating the mobility of bla. This study revealed that the bla gene is not as rare as previously found and that epidemic clones of CREC are responsible for its dissemination in China. These findings highlight the potential of wide dissemination of low-prevalence antimicrobial resistance genes.

Carbapenemase Enterobacter cloacae complex Enterobacter hormaechei Epidemic clone SFO-1

SCI

英语

第一

State Key Project of Research and Development Plan[2017YFC120020 0] ; Viral Hepatitis Prevention and Control Technology Major Projects[2018ZX10712-001] ; National Natural Science Foundation of China[81702045][81361138021] ; Shenzhen Basic Research project[JCYJ20190807144409307]

Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy

Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy

WOS:000567773900010

ELSEVIER

PHARMACOLOGY & TOXICOLOGY

2-s2.0-85089084564

Scopus

1.Shenzhen Institute of Respiratory Diseases,the First Affiliated Hospital (Shenzhen People's Hospital),Southern University of Science and Technology,and Second Clinical Medical College,Jinan University,Shenzhen,China
2.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,the First Affiliated Hospital of Medicine School,Zhejiang University,Hangzhou,China
3.Department of Laboratory Medicine,Nanjing Drum Tower Hospital,the Affiliated Hospital of Nanjing University Medical School,Nanjing,China

Zhou，Kai,Zhou，Yanzi,Zhang，Chuqiu,等. Dissemination of a ‘rare’ extended-spectrum β-lactamase gene blaSFO-1 mediated by epidemic clones of carbapenemase-producing Enterobacter hormaechei in China[J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS,2020,56(3).

Zhou，Kai.,Zhou，Yanzi.,Zhang，Chuqiu.,Song，Jingjie.,Cao，Xiaoli.,...&Xiao，Yonghong.(2020).Dissemination of a ‘rare’ extended-spectrum β-lactamase gene blaSFO-1 mediated by epidemic clones of carbapenemase-producing Enterobacter hormaechei in China.INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS,56(3).

Zhou，Kai,et al."Dissemination of a ‘rare’ extended-spectrum β-lactamase gene blaSFO-1 mediated by epidemic clones of carbapenemase-producing Enterobacter hormaechei in China".INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS 56.3(2020).