Increasing the Assembly Efficacy of Peptidic beta-Sheets for a Highly-Sensitive HIV Detection

Cao, Hongyan2,5,6; Liu, Ye1; Sun, Hongyan; Li, Zulan2,5,6; Gao, Yuan5,6; Deng, Xinli2; Shao, Yiming7; Cong, Yulong2; Jiang, Xingyu3,4,5,6

2020-08-18

10.1021/acs.analchem.0c00951

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Our recent publication illustrates the critical role of phenylalanine-mediated aromatic-aromatic interactions in determining the assembly of peptidic beta-sheets. However, the effect of phenylalanine number on regulating the assembly efficacy of peptidic beta-sheets remains poorly understood. We herein evaluate the assembly efficacy of beta-sheets of a series of oligopeptides which contain 0, 1, 2, or 3 phenylalanine in their molecular backbones. In our assembly system, two phenylalanine (2F) is the minimum number for driving the assembly of beta-sheets of oligopeptides. Oligopeptides with three phenylalanine (3F) show significantly increased assembly efficacy of beta-sheets compared to that with 2F. These results suggest a positive correlation between the phenylalanine number and assembly efficacy of beta-sheets. By improving the assembly efficacy of beta-sheets, we further develop a highly sensitive HIV analytical system in which the specific binding of beta-sheets with Congo Red induces enhanced fluorescence. For HIV p24 detection, the 3F-based analytical system (0.61 pg/mL) shows a significantly lower limit of detection (LOD) than the 2F-based analytical system (2.44 pg/mL), both of which are more sensitive than commercial ELISA (S pg/mL) used in the clinic. This work not only illustrates the effect of phenylalanine number on regulating the assembly efficacy of beta-sheets but also provides a guideline for the construction of a highly sensitive analytical system of disease diagnosis.

SCI ; EI

英语

NI论文

通讯

National Natural Science Foundation of China[21874033][21535001][81730051][21761142006] ; Leading Medical Talents Program of Health Commission of Yunnan Province[L-2018013] ; Young and Middle-Aged Academic and Technical Leaders Program of Yunnan Province, Kunming Science and Technology Bureau[2020-1-N-038] ; Ministry of Science and Technology of China[2018ZX10731101-001-011] ; Chinese Academy of Sciences[QYZDJ-SSW-SLH039][121D11KYSB20170026][XDA16020902] ; National Key R&D Program of China[2018YFA0902600][2017YFA0205901]

Chemistry

Chemistry, Analytical

WOS:000563047900031

AMER CHEMICAL SOC

20204109309337

Amino acids ; Aromatic compounds ; Azo dyes ; Diagnosis

Medicine and Pharmacology:461.6 ; Biology:461.9 ; Chemical Agents and Basic Industrial Chemicals:803 ; Organic Compounds:804.1

CHEMISTRY

Web of Science

1.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biol, Kunming 65000, Yunnan, Peoples R China
2.Peoples Liberat Army Gen Hosp, PLA Med Coll & Clin Lab, Med Ctr 2, Beijing 100853, Peoples R China
3.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Natl Ctr Nanosci & Technol, Beijing Engn Res Ctr Bionanotechnol, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China
6.Natl Ctr Nanosci & Technol, CAS Key Lab Biomed Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China
7.Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Natl Ctr AIDS STD Control & Prevent, Beijing 100190, Peoples R China

Cao, Hongyan,Liu, Ye,Sun, Hongyan,et al. Increasing the Assembly Efficacy of Peptidic beta-Sheets for a Highly-Sensitive HIV Detection[J]. ANALYTICAL CHEMISTRY,2020,92(16):11089-11094.

Cao, Hongyan.,Liu, Ye.,Sun, Hongyan.,Li, Zulan.,Gao, Yuan.,...&Jiang, Xingyu.(2020).Increasing the Assembly Efficacy of Peptidic beta-Sheets for a Highly-Sensitive HIV Detection.ANALYTICAL CHEMISTRY,92(16),11089-11094.

Cao, Hongyan,et al."Increasing the Assembly Efficacy of Peptidic beta-Sheets for a Highly-Sensitive HIV Detection".ANALYTICAL CHEMISTRY 92.16(2020):11089-11094.