Quantitative Proteomic Analyses To Reveal the Key Features of Proteins in New Onset Ankylosing Spondylitis Patients

Lu, Yong-Ping1,2; Zhang, Xiao-Li2; Zheng, Fengping3; Yun, Chen4; Zhu, Chengxin3; Cai, Wanxia3; Liu, Dongzhou3; Hong, Xiaoping3; Li, Qlang5; Hu, Bo1; Tang, Donge3; Yin, Liang-Hong1; Dai, Yong3

2020-08-18

10.1021/acsomega.0c01776

ACS Omega   影响因子和分区

2470-1343

Ankylosing spondylitis (AS) is a chronic immunemediated disease. Various immune cells play an essential role in the AS pathogenesis. However, the specific pathogenesis of AS has not been well understood. Proteomic profiles of peripheral blood mononuclear cells (PBMCs) were applied to reveal the specific pathogenesis of AS. Quantitative proteomic analyses were performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods to investigate the protein profiling of PBMCs from new-onset AS patients (n = 9) and healthy controls (n = 9). We identified 782 differentially expressed proteins (DEPs) and 527 differentially phosphorylated proteins (DPPs) between AS patients and healthy controls. The subcellular location of DEPs and DPPs showed that most of the DEPs were from the cytoplasm (n = 296, 38%), were extracellular (n = 141, 18%), and from the nucleus (n = 114, 15%); most of the DPPs were from the cytoplasm ( n = 37, 34%), nucleus (n = 35, 32%), and plasma membrane (n = 10, 9%). We further identified 89 proteins with both expression and phosphorylation differences. The functional annotation of the 89 differentially expressed and phosphorylated proteins enriched in the antigen processing and presentation pathway. Four DEPs with six phosphorylated positions were found in the antigen processing and presentation pathway. The differentially expressed and phosphorylated proteins may be helpful to uncover the pathogenesis of AS. The six AS-specific proteins may serve as candidate markers for AS diagnosis and new treatment targets.

SCI

英语

通讯

Key Research and Development Program of Guangdong Province[2019B020229001] ; Science and Technology Planning Project of Guangdong Province[2017B020209001] ; National Natural Science Foundation of China[81671596] ; science and technology plan of Shenzhen[JCYJ20170307095606266] ; Postdoctoral Fund of the First Affiliated Hospital, Jinan University[809001] ; Young Innovative Talents Project of General Colleges and Universities in Guangdong Province[2018KQNCX010]

Chemistry

Chemistry, Multidisciplinary

WOS:000563015100024

AMER CHEMICAL SOC

Web of Science

1.Jinan Univ, Affiliated Hosp 1, Dept Nephrol, Guangzhou 510632, Peoples R China
2.Heidelberg Univ, Univ Med Ctr Mannheim, Dept Med Nephrol Endocrinol Rheumatol 5, DE-68135 Mannheim, Baden Widttembe, Germany
3.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2, Shenzhen 518020, Peoples R China
4.Charite Univ Med Berlin, Dept Nephrol, DE-10117 Berlin, Germany
5.Dongguan Hosp Tradit Chinese Med, Dept Nephrol, Dongguan 523000, Peoples R China

Lu, Yong-Ping,Zhang, Xiao-Li,Zheng, Fengping,et al. Quantitative Proteomic Analyses To Reveal the Key Features of Proteins in New Onset Ankylosing Spondylitis Patients[J]. ACS Omega,2020,5(32):20153-20161.

Lu, Yong-Ping.,Zhang, Xiao-Li.,Zheng, Fengping.,Yun, Chen.,Zhu, Chengxin.,...&Dai, Yong.(2020).Quantitative Proteomic Analyses To Reveal the Key Features of Proteins in New Onset Ankylosing Spondylitis Patients.ACS Omega,5(32),20153-20161.

Lu, Yong-Ping,et al."Quantitative Proteomic Analyses To Reveal the Key Features of Proteins in New Onset Ankylosing Spondylitis Patients".ACS Omega 5.32(2020):20153-20161.