Identification of novel tetracycline resistance genetet(X14) and its co-occurrence withtet(X2) in a tigecycline-resistant and colistin-resistantEmpedobacter stercoris

Cheng, Yingying1,2; Chen, Yong1,2; Liu, Yang1,2; Guo, Yuqi1,2; Zhou, Yanzi3; Xiao, Tingting3; Zhang, Shuntian3; Xu, Hao3; Chen, Yunbo3; Shan, Tongling4; Xiao, Yonghong3; Zhou, Kai1,2

2020-01

10.1080/22221751.2020.1803769

Emerging Microbes & Infections   影响因子和分区

2222-1751

2222-1751

Tigecycline is one of the last-resort antibiotics to treat severe infections. Recently, tigecycline resistance has sporadically emerged with an increasing trend, and Tet(X) family represents a new resistance mechanism of tigecycline. In this study, a novel chromosome-encoded tigecycline resistance gene,tet(X14), was identified in a tigecycline-resistant and colistin-resistantEmpedobacter stercorisstrain ES183 recovered from a pig fecal sample in China. Tet(X14) shows 67.14-96.39% sequence identity to the other variants [Tet(X) to Tet(X13)]. Overexpression of Tet(X14) inEscherichia coliconfers 16-fold increase in tigecycline MIC (from 0.125 to 2 mg/L), which is lower than that of Tet(X3), Tet(X4) and Tet(X6). Structural modelling predicted that Tet(X14) shared a high homology with the other 12 variants with RMSD value from 0.003 to 0.055, and Tet(X14) can interact with tetracyclines by a similar pattern as the other Tet(X)s.tet(X14) and two copies oftet(X2) were identified on a genome island with abnormal GC content carried by the chromosome of ES183, and no mobile genetic elements were found surrounding, suggesting thattet(X14) might be heterologously obtained by ES183 via recombination. Blasting in Genbank revealed that Tet(X14) was exclusively detected on the chromosome ofRiemerella anatipestifer, mainly encoded on antimicrobial resistance islands.E. stercorisandR. anatipestiferbelong to the familyFlavobacteriaceae, suggesting that the members ofFlavobacteriaceaemaybe the major reservoir oftet(X14). Our study reports a novel chromosome-encoded tigecycline resistance genetet(X14). The expanded members of Tet(X) family warrants the potential large-scale dissemination and the necessity of continuous surveillance fortet(X)-mediated tigecycline resistance.

Tigecycline resistance tetracycline resistance tet(X14) tet(X2) Empedobacter stercoris

SCI

英语

通讯

National Key Research and Development Program of China[2017YFC1200200] ; Major Infectious Diseases Such as AIDS and Viral Hepatitis Prevention and Control Technology Major Projects[2018ZX10712-001] ; National Natural Science Foundation of China[81702045][81902029] ; Shenzhen Basic Research projects[JCYJ20190807144409307][JCYJ20190807150401657]

Immunology ; Microbiology

Immunology ; Microbiology

WOS:000561560200001

TAYLOR & FRANCIS LTD

Web of Science

1.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Shenzhen Inst Resp Dis, Dongmen North Rd 1017, Shenzhen 518020, Peoples R China
2.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Shenzhen, Peoples R China
3.Zhejiang Univ, Coll Med, Collaborat Innovat Ctr Diag & Treatment Infect Di, Affiliated Hosp 1,State Key Lab Diag & Treatment, Hangzhou, Peoples R China
4.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai, Peoples R China

Cheng, Yingying,Chen, Yong,Liu, Yang,et al. Identification of novel tetracycline resistance genetet(X14) and its co-occurrence withtet(X2) in a tigecycline-resistant and colistin-resistantEmpedobacter stercoris[J]. Emerging Microbes & Infections,2020,9(1):1843-1852.

Cheng, Yingying.,Chen, Yong.,Liu, Yang.,Guo, Yuqi.,Zhou, Yanzi.,...&Zhou, Kai.(2020).Identification of novel tetracycline resistance genetet(X14) and its co-occurrence withtet(X2) in a tigecycline-resistant and colistin-resistantEmpedobacter stercoris.Emerging Microbes & Infections,9(1),1843-1852.

Cheng, Yingying,et al."Identification of novel tetracycline resistance genetet(X14) and its co-occurrence withtet(X2) in a tigecycline-resistant and colistin-resistantEmpedobacter stercoris".Emerging Microbes & Infections 9.1(2020):1843-1852.