Combinatory Data-Independent Acquisition and Parallel Reaction Monitoring Method for Deep Profiling of Gangliosides

Li, Hua2,3; Xu, Ruilian4,5; Yang, Lijun1,4,5; Luan, Hemi6; Chen, Shili7; Chen, Lan1; Cai, Zongwei2; Tian, Ruijun1

2020-08-04

10.1021/acs.analchem.0c02313

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Ganglioside is an important class of lipid species involved in intercellular signaling and various diseases, especially for neurodegenerative diseases. Systematic ganglioside profiling is challenging because of their naturally low abundance and highly diverse species. Herein, a new data-independent acquisition and parallel reaction monitoring (DIA/PRM) method with superior sensitivity was developed. The untargeted DIA acquisition consecutively records all the precursor ion and fragment ions at the same time, while the targeted PRM analysis with versatile higher collisional dissociation generates full MS/MS spectra for structure elucidation and verification. As compared with traditional data-dependent acquisition (DDA), the DIA/PRM method unbiasedly detected the majority of abundant ganglioside species and as low as 50 pg of ganglioside in an untargeted manner. Gangliosides in four kinds of biological samples including the mouse brain, mouse plasma, HeLa cell, and human colon cancer tissue were systematically identified, and low-abundance ganglioside species were further extended on the basis of linear chromatography retention rules of the most frequently detected ganglioside species. A total of 383 ganglioside features were defined with 329 of them derived from 32 ganglioside species. Taking advantage of the high-resolution MS analysis, rare ganglioside species were further elucidated according to their characteristic fragment ions and neutral losses. In total, 18 gangliosides with a ceramide carbon number from 20 to 25 and modified gangliosides, including 18 acetylated, 8 diacetylated, 1 phosphorylated, 36 N-glycolyneuraminic acid (NeuGc)-containing, and 7 di-NeuGc-containing gangliosides, were newly identified. The developed DIA/PRM method therefore generated a rich ganglioside resource for further functional exploration and is a unique alternative for DDA analysis for global ganglioside profiling in various biological systems.

SCI ; EI

英语

NI论文

通讯

National Natural Science Foundation of China[21904057][91953118][21904058][21705108]

Chemistry

Chemistry, Analytical

WOS:000558761500082

AMER CHEMICAL SOC

20203809190961

Ions ; Chromatography ; Lanthanum compounds

Medicine and Pharmacology:461.6

CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Coll Sci, Dept Chem, Shenzhen 518055, Peoples R China
2.Hong Kong Baptist Univ, Dept Chem, State Key Lab Environm & Biol Anal, Hong Kong, Peoples R China
3.Southern Univ Sci & Technol, SUSTech Core Res Facil, Shenzhen 518055, Peoples R China
4.SUSTech, Affiliated Hosp 1, Dept Oncol, Shenzhen 518020, Peoples R China
5.Shenzhen Peoples Hosp, Shenzhen 518020, Peoples R China
6.Southern Univ Sci & Technol, SUSTech Acad Adv Interdisciplinary Studies, Shenzhen 518055, Peoples R China
7.Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Gen Surg, Shanghai 200092, Peoples R China

Li, Hua,Xu, Ruilian,Yang, Lijun,et al. Combinatory Data-Independent Acquisition and Parallel Reaction Monitoring Method for Deep Profiling of Gangliosides[J]. ANALYTICAL CHEMISTRY,2020,92(15):10830-10838.

Li, Hua.,Xu, Ruilian.,Yang, Lijun.,Luan, Hemi.,Chen, Shili.,...&Tian, Ruijun.(2020).Combinatory Data-Independent Acquisition and Parallel Reaction Monitoring Method for Deep Profiling of Gangliosides.ANALYTICAL CHEMISTRY,92(15),10830-10838.

Li, Hua,et al."Combinatory Data-Independent Acquisition and Parallel Reaction Monitoring Method for Deep Profiling of Gangliosides".ANALYTICAL CHEMISTRY 92.15(2020):10830-10838.