CLIC2 alpha Chloride Channel Orchestrates Immunomodulation of Hemocyte Phagocytosis and Bactericidal Activity in Crassostrea gigas

Zhang, Xiangyu1,2,5; Mao, Fan1,2; Wong, Nai-Kei1,3; Bao, Yongbo4; Lin, Yue1,2,5; Liu, Kunna1,2,5; Li, Jun1,2; Xiang, Zhiming1,2; Ma, Haitao1,2; Xiao, Shu1,2; Zhang, Yang1,2; Yu, Ziniu1,2

2020-07-24

10.1016/j.isci.2020.101328

iScience   影响因子和分区

2589-0042

2589-0042

Chloride ion plays critical roles in modulating immunological interactions. Herein, we demonstrated that the anion channel CLIC2 alpha mediates Cl- flux to regulate hemocytes functions in the Pacific oyster (Crassostrea gigas). Specifically, during infection by Vibrio parahemolyticus, chloride influx was activated following onset of phagocytosis. Phosphorylation of Akt was stimulated by Cl- ions entering host cells, further contributing to signal transduction regulating internalization of bacteria through the PI3K/Akt signaling pathway. Concomitantly, Cl- entered phagosomes, promoted the acidification and maturation of phagosomes, and contributed to production of HOCl to eradicate engulfed bacteria. Finally, genomic screening reveals CLIC2 alpha as a major Cl- channel gene responsible for regulating Cl- influx in oysters. Knockdown of CLIC2 alpha predictably impeded phagosome acidification and restricted bacterial killing in oysters. In conclusion, our work has established CLIC2 alpha as a prominent regulator of Cl- influx and thus Cl- function in C. gigas in bacterial infection contexts.

SCI

英语

其他

National Science Foundation of China[31902404] ; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[GML2019ZD0407] ; China Agricultural Research System[CARS-49] ; Science and Technology Program of Guangzhou, China[201804020073] ; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China[201806010003] ; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences[ISEE2018PY01][ISEE2018PY03][ISEE2018ZD01] ; Science and Technology Planning Project of Guangdong Province, China[2017B030314052][201707010177] ; Demonstration Project for Innovative Development of Marine Economy[NBHY-2017-S4]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000555586700001

CELL PRESS

Web of Science

1.Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, CAS Key Lab Trop Marine Bio Resources & Ecol, Guangdong Prov Key Lab Appl Marine Biol,South Chi, Guangzhou 510301, Peoples R China
2.Southern Marine Sci & Engn Guangdong Lab Guangzho, Guangzhou 510301, Peoples R China
3.Southern Univ Sci & Technol, Natl Clin Res Ctr Infect Dis, Shenzhen Peoples Hosp 3, Hosp 2, Shenzhen 518112, Peoples R China
4.Zhejiang Wanli Univ, Coll Biol & Environm Sci, Zhejiang Key Lab Aquat Germplasm Resources, Ningbo 315100, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Zhang, Xiangyu,Mao, Fan,Wong, Nai-Kei,et al. CLIC2 alpha Chloride Channel Orchestrates Immunomodulation of Hemocyte Phagocytosis and Bactericidal Activity in Crassostrea gigas[J]. iScience,2020,23(7):1-+.

Zhang, Xiangyu.,Mao, Fan.,Wong, Nai-Kei.,Bao, Yongbo.,Lin, Yue.,...&Yu, Ziniu.(2020).CLIC2 alpha Chloride Channel Orchestrates Immunomodulation of Hemocyte Phagocytosis and Bactericidal Activity in Crassostrea gigas.iScience,23(7),1-+.

Zhang, Xiangyu,et al."CLIC2 alpha Chloride Channel Orchestrates Immunomodulation of Hemocyte Phagocytosis and Bactericidal Activity in Crassostrea gigas".iScience 23.7(2020):1-+.