Electrochemical sensor based on dual-template molecularly imprinted polymer and nanoporous gold leaf modified electrode for simultaneous determination of dopamine and uric acid

Li, Nan1,2; Nan, Chuanchuan2,3; Mei, Xuecui4; Sun, Yudong4; Feng, Huanhuan5,6; Li, Yingchun4,7

2020-08-15

10.1007/s00604-020-04413-5

MICROCHIMICA ACTA   影响因子和分区

0026-3672

1436-5073

A novel electrochemical sensor based on dual-template molecularly imprinted polymer (MIP) with nanoporous gold leaf (NPGL) was established for the simultaneous determination of dopamine (DA) and uric acid (UA). NPGL acts as an enlarged loading platform to enhance sensing capacity, and the MIP layer was synthesized in situ in the presence of monomer and dual templates (DA and UA) to provide specific recognition. Under the optimal conditions, the sensor shows a good linear range of 2.0 similar to 180 mu M for DA at a working potential of 0.15 V (vs. Ag/AgCl) and 5.0 similar to 160 mu M for UA at 0.35 V (vs. Ag/AgCl), with the respective detection limit of 0.3 mu M and 0.4 mu M (S/N=3). Good selectivity of the sensor to its dual templates was confirmed as the sensing signals are significantly different between templates and interfering species. The responses maintained higher than 96% of the initial values after 30-day storage, and the day-to-day relative standard deviation is less than 3.0%. Real sample simultaneous determination of DA and UA was conducted with bovine serum, and the results were in good agreement with those from high-performance liquid chromatography. It can be concluded that this work offers a reliable, facile, fast, and cost-effective method of simultaneous quantification of two or more chem-/bio-molecules.

Molecular imprinting technique Nanomaterial Biomarker Electro-polymerization Multiple targets

SCI

英语

其他

Shenzhen science and technology research and development fund[JCYJ20180305180609083] ; Shenzhen Science and Technology Program[KQJSCX20180328165437711][KQTD20170810105439418] ; project on the key technique improvement of Xinjiang Licorice planting and quality control of Xinjiang Production & Construction Corps[2018AB012] ; National Natural Science Foundation of China[81773680][81973280] ; Innovative team and talent training project of Shihezi[2018TD02]

Chemistry

Chemistry, Analytical

WOS:000563558500001

SPRINGER WIEN

CHEMISTRY

Web of Science

1.Jinan Univ, Stomatol Ctr, Shenzhen Peoples Hosp, Clin Med Sch 2, Shenzhen 518020, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
3.Jinan Univ, Dept ICU, Shenzhen Peoples Hosp, Clin Med Sch 2, Shenzhen 518020, Guangdong, Peoples R China
4.Harbin Inst Technol Shenzhen, Coll Sci, Shenzhen 518055, Guangdong, Peoples R China
5.Harbin Inst Technol Shenzhen, Sch Mat Sci & Engn, Res Ctr Printed Flexible Elect, Shenzhen 518055, Peoples R China
6.Harbin Inst Technol Shenzhen, State Key Lab Adv Welding & Joining, Shenzhen 518055, Peoples R China
7.Shenzhen Univ, Coll Optoelect Engn, Shenzhen 518060, Peoples R China

Li, Nan,Nan, Chuanchuan,Mei, Xuecui,et al. Electrochemical sensor based on dual-template molecularly imprinted polymer and nanoporous gold leaf modified electrode for simultaneous determination of dopamine and uric acid[J]. MICROCHIMICA ACTA,2020,187(9).

Li, Nan,Nan, Chuanchuan,Mei, Xuecui,Sun, Yudong,Feng, Huanhuan,&Li, Yingchun.(2020).Electrochemical sensor based on dual-template molecularly imprinted polymer and nanoporous gold leaf modified electrode for simultaneous determination of dopamine and uric acid.MICROCHIMICA ACTA,187(9).

Li, Nan,et al."Electrochemical sensor based on dual-template molecularly imprinted polymer and nanoporous gold leaf modified electrode for simultaneous determination of dopamine and uric acid".MICROCHIMICA ACTA 187.9(2020).