Elevated intracellular cyclic-di-GMP level inShewanella oneidensisincreases expression ofc-type cytochromes

Ng, Chun Kiat1,2; Xu, Jiabao1; Cai, Zhao2,4; Yang, Liang2; Thompson, Ian P.1; Huang, Wei E.1; Cao, Bin2,3

2020-07-30

10.1111/1751-7915.13636

MICROBIAL BIOTECHNOLOGY   影响因子和分区

1751-7915

Electrochemically active biofilms are capable of exchanging electrons with solid electron acceptors and have many energy and environmental applications such as bioelectricity generation and environmental remediation. The performance of electrochemically active biofilms is usually dependent onc-type cytochromes, while biofilm development is controlled by a signal cascade mediated by the intracellular secondary messenger bis-(3MODIFIER LETTER PRIME-5MODIFIER LETTER PRIME) cyclic dimeric guanosine monophosphate (c-di-GMP). However, it is unclear whether there are any links between the c-di-GMP regulatory system and the expression ofc-type cytochromes. In this study, we constructed aS. oneidensisMR-1 strain with a higher cytoplasmic c-di-GMP level by constitutively expressing a c-di-GMP synthase and it exhibited expected c-di-GMP-influenced traits, such as lowered motility and increased biofilm formation. Compared to MR-1 wild-type strain, the high c-di-GMP strain had a higher Fe(III) reduction rate (21.58 vs 11.88 pM of Fe(III)/h cell) and greater expression of genes that code for the proteins involved in the Mtr pathway, including CymA, MtrA, MtrB, MtrC and OmcA. Furthermore, single-cell Raman microspectroscopy (SCRM) revealed a great increase ofc-type cytochromes in the high c-di-GMP strain as compared to MR-1 wild-type strain. Our results reveal for the first time that the c-di-GMP regulation system indirectly or directly positively regulates the expression of cytochromes involved in the extracellular electron transport (EET) inS. oneidensis, which would help to understand the regulatory mechanism of c-di-GMP on electricity production in bacteria.

SCI

英语

其他

Ministry of Education (MOE) Academic Research Fund (AcRF) Tier 2 Grant[MOE2017-T2-2-042] ; MOE Singapore under its Research Centre of Excellence Programme, Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore[M4330005.C70] ; EPSRC[EP/M002403/1][EP/M02833X/1] ; NERC[NE/M002934/1] ; Commonwealth Scholarship Commission[SGRF-2017-471]

Biotechnology & Applied Microbiology ; Microbiology

Biotechnology & Applied Microbiology ; Microbiology

WOS:000553395400001

WILEY

Web of Science

1.Univ Oxford, Dept Engn Sci, Parks Rd, Oxford OX1 3PJ, England
2.Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore, Singapore
3.Nanyang Technol Univ, Sch Civil & Environm Engn, Singapore, Singapore
4.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China

Ng, Chun Kiat,Xu, Jiabao,Cai, Zhao,et al. Elevated intracellular cyclic-di-GMP level inShewanella oneidensisincreases expression ofc-type cytochromes[J]. MICROBIAL BIOTECHNOLOGY,2020,13(6):1904-1916.

Ng, Chun Kiat.,Xu, Jiabao.,Cai, Zhao.,Yang, Liang.,Thompson, Ian P..,...&Cao, Bin.(2020).Elevated intracellular cyclic-di-GMP level inShewanella oneidensisincreases expression ofc-type cytochromes.MICROBIAL BIOTECHNOLOGY,13(6),1904-1916.

Ng, Chun Kiat,et al."Elevated intracellular cyclic-di-GMP level inShewanella oneidensisincreases expression ofc-type cytochromes".MICROBIAL BIOTECHNOLOGY 13.6(2020):1904-1916.