南方科技大学知识苑(SUSTech KC): Chemoproteomic Profiling of Cobalamin-Independent Methionine Synthases in Plants with a Covalent Probe

题名	Chemoproteomic Profiling of Cobalamin-Independent Methionine Synthases in Plants with a Covalent Probe
作者	Chen, Xin 1,2; Xu, Jingyuan 1; Wong, Nai-Kei 3; Zhong, Suyun 1; Yang, Mengquan 2; Liu, Zhen 1; Lu, Yan 1; Li, Weichao 2; Zhou, Yiqing 1,2
通讯作者	Zhou, Yiqing
发表日期	2020-07-29
DOI	10.1021/acs.jafc.0c03301
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 影响因子和分区
ISSN	0021-8561
EISSN	1520-5118
卷号	68 期号:30页码:8050-8056
摘要	Cobalamin-independent methionine synthases (MS) are zinc-binding methyltransferases that catalyze de novo methionine biosynthesis in higher plants, which are enzymes critically involved in seed germination and plant growth. Here, we report a highly selective sulfonyl fluoride-based probe for chemoproteomic profiling of MS enzymes in living systems of the model plant Arabidopsis thaliana, as implemented in in-gel-, mass spectrometry-, and imaging-based platforms. This probe holds promise for facilitating and accelerating fundamental research and industrial application of MS enzymes, particularly in the contexts of MS1/2-targeting herbicide screening and design.
关键词	cobalamin-independent methionine synthases chemical probe covalent chemoproteomics herbicide
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
学校署名	其他
资助项目	Natural Science Foundation of Jiangsu Province of China[BK20191477] ; Natural Science Foundation of the Higher Education Institutions of Jiangsu Province of China[19KJB150001] ; Natural Science Foundation of China[21502206][21807102]
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS记录号	WOS:000557855900023
出版者	AMER CHEMICAL SOC
ESI学科分类	AGRICULTURAL SCIENCES
来源库	Web of Science
引用统计	被引频次[WOS]：2
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/186754
专题	南方科技大学第二附属医院南方科技大学第一附属医院
作者单位	1.Changshu Inst Technol, Sch Biotechnol & Food Engn, Changshu 215500, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Plant Physiol & Ecol, CAS Key Lab Synthet Biol, Shanghai 200032, Peoples R China 3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Hosp Affiliated 2, Shenzhen 518112, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xin,Xu, Jingyuan,Wong, Nai-Kei,et al. Chemoproteomic Profiling of Cobalamin-Independent Methionine Synthases in Plants with a Covalent Probe[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2020,68(30):8050-8056.
APA	Chen, Xin.,Xu, Jingyuan.,Wong, Nai-Kei.,Zhong, Suyun.,Yang, Mengquan.,...&Zhou, Yiqing.(2020).Chemoproteomic Profiling of Cobalamin-Independent Methionine Synthases in Plants with a Covalent Probe.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,68(30),8050-8056.
MLA	Chen, Xin,et al."Chemoproteomic Profiling of Cobalamin-Independent Methionine Synthases in Plants with a Covalent Probe".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 68.30(2020):8050-8056.