Delayed application of silver nanoparticles reveals the role of early inflammation in burn wound healing

Zhang, Kangjun1,4; Lui, Vincent C. H.1; Chen, Yan2; Lok, Chun Nam3; Wong, Kenneth K. Y.1

2020-04-14

10.1038/s41598-020-63464-z

Scientific Reports   影响因子和分区

2045-2322

Burn injury is common, and antimicrobial agents are often applied immediately to prevent wound infection and excessive inflammatory response. Although inflammation is essential for clearing bacteria and creating an environment conducive to the healing process, it is unclear what time-frame inflammation should be present for optimal wound healing. This study critically investigated the role of early inflammation in burn wound healing, and also revealed the molecular mechanisms underlying the pro-healing effects of silver nanoparticles (AgNPs). We created a burn injury mouse model using wild-type and Smad3-/- mice, which were topically treated with AgNPs at different post-burn days, and examined the healing processes of the various groups. We also delineated the molecular pathways underlying the anti-inflammation and pro-healing effects of AgNPs by morphological and histological analysis, immuno-histochemistry, and western blotting. Our results showed that (1) AgNPs regulated pro-inflammatory cytokine IL-6 production of keratinocytes and neutrophils infiltration through KGF-2/p38 signaling pathway, (2) Topical AgNPs treatment immediately after burn injury significantly supressed early inflammation but resulted in delayed healing, (3) A short delay in AgNPs application (post-burn day 3 in our model) allowed early inflammation in a controlled manner, and led to optimal burn wound healing. Thus, our current study showed that some degree of early inflammation was beneficial, but prolonged inflammation was detrimental for burn wound healing. Further evaluation and clinical translation of this finding is warranted.

SCI

英语

其他

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000562162800016

NATURE PUBLISHING GROUP

Web of Science

1.Univ Hong Kong, LKS Fac Med, Dept Surg, Hong Kong, Peoples R China
2.Guangzhou Women & Childrens Med Ctr, Dept Surg, Guangzhou, Peoples R China
3.Univ Hong Kong, Fac Sci, Dept Chem, Hong Kong, Peoples R China
4.Southern Univ Sci & Technol, Affiliated Hosp 2, Peoples Hosp Shenzhen 3, Shenzhen, Peoples R China

Zhang, Kangjun,Lui, Vincent C. H.,Chen, Yan,et al. Delayed application of silver nanoparticles reveals the role of early inflammation in burn wound healing[J]. Scientific Reports,2020,10(1).

Zhang, Kangjun,Lui, Vincent C. H.,Chen, Yan,Lok, Chun Nam,&Wong, Kenneth K. Y..(2020).Delayed application of silver nanoparticles reveals the role of early inflammation in burn wound healing.Scientific Reports,10(1).

Zhang, Kangjun,et al."Delayed application of silver nanoparticles reveals the role of early inflammation in burn wound healing".Scientific Reports 10.1(2020).